SELECTIVE BLOCKING OF POLYMERIZATION REACTION OF STRIATED-MUSCLE ACTIN LEADING TO A DERIVATIVE SUITABLE FOR CRYSTALLIZATION - MODIFICATION OF TYR-53 BY 5-DIAZONIUM-(1H)TETRAZOLE
SELECTIVE BLOCKING OF POLYMERIZATION REACTION OF STRIATED-MUSCLE ACTIN LEADING TO A DERIVATIVE SUITABLE FOR CRYSTALLIZATION - MODIFICATION OF TYR-53 BY 5-DIAZONIUM-(1H)TETRAZOLE
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DOI：
10.1111/j.1432-1033.1976.tb10290.x
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发表时间：
1976-01-01
期刊：
影响因子：
--
通讯作者：
VOLK, KE
中科院分区：
文献类型：
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作者：
BENDER, N;FASOLD, H;VOLK, KE
1个氨基酸侧链/蛋白质单体与5-重氮-(1H)[14C]四唑的反应完全抑制了兔肌肉肌动蛋白的聚合反应。胰蛋白酶肽指纹显示由试剂标记的单个肽。分离肽并通过氨基酸分析鉴定标记的氨基酸为Tyr-53。 F-肌动蛋白中的试剂无法接触到该侧链。将修饰与其他试剂的类似抑制进行比较。
The polymerization reaction of rabbit muscle actin was completely inhibited by reaction of 1 amino acid side chain/protein monomer with 5-diazonium-(1H)[14C]tetrazole. A tryptic peptide fingerprint showed a single peptide labeled by the reagent. The peptide was isolated and the labeled amino acid identified by amino acid analysis as Tyr-53. This side chain is not accessible to the reagent in F-actin. The modification is compared to similar inhibitions by other reagents.