Simian virus 40 inhibits differentiation and maturation of rhesus macaque DC-SIGN(+) dendritic cells.
Simian virus 40 inhibits differentiation and maturation of rhesus macaque DC-SIGN(+) dendritic cells.
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DOI：
10.1186/2047-783x-15-9-377
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发表时间：
2010-09-24
影响因子：
4.2
通讯作者：
Wu NP
中科院分区：
文献类型：
--
作者：
Changyong C;Sun M;Li H;Brockmeyer N;Wu NP
树突状细胞（DC）是免疫应答的启动者和调节者。一些病原微生物通过控制DC的抗原提呈功能，发展出免疫逃避策略。猿猴病毒40（Simian virus 40，SV 40）是一种恒河猴源性DNA肿瘤病毒。它可以在许多脊椎动物物种中诱导细胞转化和肿瘤发生，但在自然条件下通常不会引起明显的影响，并且在恒河猴中作为潜伏感染持续存在。为探讨SV 40与恒河猴DC的相互作用，用重组人白细胞介素4（rhIL-4）和感染性SV 40诱导恒河猴外周血单核细胞来源DC，用流式细胞术（FCM）和混合淋巴细胞反应（MLR）分析DC特异性细胞内粘附分子3（DC-SIGN）+ DC的表型和功能。结果表明，SV 40可下调DC表面CD 83和CD 86的表达，抑制DC诱导的T细胞增殖。这些结果表明，SV 40也可能通过影响DC的分化和成熟而引起免疫抑制。
Dendritic cells (DC) are the initiators and modulators of the immune responses. Some species of pathogenic microorganisms have developed immune evasion strategies by controlling antigen presentation function of DC. Simian virus 40 (SV40) is a DNA tumor virus of rhesus monkey origin. It can induce cell transformation and tumorigenesis in many vertebrate species, but often causes no visible effects and persists as a latent infection in rhesus monkeys under natural conditions. To investigate the interaction between SV40 and rhesus monkey DC, rhesus monkey peripheral blood monocyte-derived DC were induced using recombinant human Interleukin-4 (rhIL-4) and infective SV40, the phenotype and function of DC-specific intracellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN)+ DC were analyzed by flow cytometry (FCM) and mixed lymphocyte reaction (MLR). Results showed that SV40 can down-regulate the expression of CD83 and CD86 on DC and impair DC-induced activation of T cell proliferation. These findings suggest that SV40 might also cause immune suppression by influencing differentiation and maturation of DC.