An intrinsically protein-resistant surface plasmon resonance biosensor based upon a RF-plasma-deposited thin film
An intrinsically protein-resistant surface plasmon resonance biosensor based upon a RF-plasma-deposited thin film
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DOI：
10.1016/s0925-4005(98)00330-x
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发表时间：
1999-01-25
影响因子：
8.4
通讯作者：
Yee, SS
中科院分区：
文献类型：
--
作者：
Mar, MN;Ratner, BD;Yee, SS
介绍了用于表面等离子共振 (SPR) 生物传感器的防污化学覆盖层的开发。三甘醇二甲醚从射频辉光放电等离子体反应器沉积到 SPR 基底上。产生的薄膜富含聚环氧乙烷基团，已知这些基团可以抵抗生物分子的吸收。利用 X 射线光电子能谱和傅里叶变换红外光谱来识别和验证薄膜成分。该薄膜在平面 SPR 装置中表现出对牛血清白蛋白污染的高度抵抗力。这些结果表明等离子体沉积的三甘醇二甲醚有望改性先前因生物污垢而受损的底物。 (C) 1999 Elsevier Science S.A. 保留所有权利。
The development of a nonfouling chemical overlayer for a surface plasmon resonance (SPR) biosensor is presented. Triethylene glycol dimethyl ether was deposited onto SPR substrates from a radio frequency glow discharge plasma reactor. A thin film was produced that is rich in poly(ethylene oxide) groups that are known to resist biomolecule uptake. X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy were utilized to identify and verify film composition. The films are shown to be highly resistant to bovine serum albumin fouling in a planar SPR apparatus. These results indicate the promise of plasma-deposited triglyme to modify substrates previously compromised by biological fouling. (C) 1999 Elsevier Science S.A. Al rights reserved.