Construction of a multicoaxial hollow fiber bioreactor.
Construction of a multicoaxial hollow fiber bioreactor.
复制标题
多同轴中空纤维生物反应器的构建。
DOI：
10.1007/978-1-62703-363-3_19
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Macdonald,JeffreyM
中科院分区：
文献类型：
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作者：
McClelland,Randall;Tech,Katherine;Macdonald,JeffreyM
生物反应器是一种组合工具，旨在利用具有内在生物学相关性的工程原理。这样的反应堆是作为体外模型创建的，以更好地复制体内自然器官。这些生物工具是跨学科组织工程领域的子集，作为惰性设备而建立，以改善生物刺激，同时允许非破坏性地测量组织功能特性。设计和制造工作的重点是二维(2D)和三维(3D)物理结构，同时将环境-细胞关系和微环境联系起来。产品的熟练程度通常涉及材料支架、营养分散、分隔单元、被动和动态流动通道、温度调节、压力管理，以及来自各种器官的细胞系或原代细胞作为组织。生物反应器的进展继续遵循跨学科的原则，如节能、细胞生态系统、系统生物学方法和活细胞设计创新。在这里，我们描述了用于肝细胞的中空纤维多同轴生物反应器的设计和构造，该中空纤维多同轴生物反应器具有整体充氧(即在生物反应器内充氧)，但它可以用于任何依赖锚定的细胞类型。
Bioreactors are assembled tools conceived to exploit engineering principles with inbuilt biological relevance. Such reactors are created as in vitro models to better replicate natural in vivo organs. These biotools are subsets within the interdisciplinary tissue engineering field and are established as inert devices to improve upon biological stimuli while simultaneously allowing tissue functional properties to be nondestructively measured. Design and fabrication efforts are focused on two-dimensional (2D) and three-dimensional (3D) physical constructs while linking environment–cell relations, the microenvironment. Product proficiencies generally involve material scaffolds, nutrient dispersion, compartmentalized units, passive and kinetic flow channels, temperature regulation, pressure management, and cell line or primary cells from assorted organs as tissues. Bioreactor advancements continue with interdisciplinary principles such as energy conservation, cell ecosystems, system-biological approaches, and viable-cell design innovation. Herein, we describe the design and construction of a hollow fiber multicoaxial bioreactor with integral oxygenation (i.e., oxygenation within the bioreactor proper) for use with liver cells, but it could be used with any anchorage-dependent cell type.