PTC IS A NOVEL REARRANGED FORM OF THE RET PROTO-ONCOGENE AND IS FREQUENTLY DETECTED INVIVO IN HUMAN THYROID PAPILLARY CARCINOMAS
PTC IS A NOVEL REARRANGED FORM OF THE RET PROTO-ONCOGENE AND IS FREQUENTLY DETECTED INVIVO IN HUMAN THYROID PAPILLARY CARCINOMAS
复制标题
DOI：
10.1016/0092-8674(90)90659-3
复制
发表时间：
1990-02-23
期刊：
影响因子：
64.5
通讯作者：
VECCHIO, G
中科院分区：
文献类型：
--
作者：
GRIECO, M;SANTORO, M;VECCHIO, G
我们最近通过对20例原发性人甲状腺乳头状癌中的5例和淋巴结转移的NIH 3T3细胞进行转染分析，发现了一种新的激活的癌基因。我们将这种转化基因命名为PTC（用于甲状腺乳头状癌）。这里我们描述了基因的分子克隆和测序。新的癌基因是由ret原癌基因酪氨酸激酶结构域的未知氨基末端序列重排引起的。在所有的转染物和所有的原始肿瘤DNA中都检测到这种基因重排，但在同一患者的正常DNA中没有检测到这种基因重排，从而表明这种遗传病变发生在体内，并且是特异性的躯体肿瘤。此外，融合基因编码的转录本在另一个ptc阳性的人乳头状癌中检测到，其mRNA可用。
We recently detected a novel activated onocogene by transfection analysis on NIH 3T3 cells in five out of 20 primary human thyroid papillary carcinomas and in the available lymph node metastases. We designated this transforming gene PTC (for papillary thyroid carcinoma). Here we describe the molecular cloning and sequencing of the gene. The new oncogene resulted from the rearrangement of an unknown amino-terminal sequence to the tyrosine kinase domain of the ret proto-oncogene. This gene rearrangement was detected in all of the transfectants and all of the original tumor DNAs, but not in normal DNA of the same patients, thus indicating that this genetic lesion occurred in vivo and is specific to somatic tumors. Moreover, the transcript coded for by the fused gene was detected in an additional PTC-positive human papillary carcinoma for which mRNA was available.