Differential expressions of BMP family genes during chondrogenic differentiation of mouse ATDC5 cells
Differential expressions of BMP family genes during chondrogenic differentiation of mouse ATDC5 cells
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DOI：
10.1247/csf.25.195
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发表时间：
2000-06-01
影响因子：
1.5
通讯作者：
Hiraki, Y
中科院分区：
文献类型：
--
作者：
Akiyama, H;Shukunami, C;Hiraki, Y
克隆细胞系ATDC5能够在单次培养中监测早期和晚期软骨分化，未分化的ATDC5细胞通过细胞凝聚阶段（早期分化）分化为表达ii型胶原的软骨细胞，然后分化为表达X型胶原的肥厚软骨细胞（晚期分化）。骨形态蛋白（BMP）信号的激活加速了细胞分化的进程。ATDC5细胞表达至少四个BMP家族成员的转录本。BMP- 1转录本在分化的所有阶段都有表达，BMP 1A型受体（ALK-3）和BMP II型受体的转录本也是如此。相比之下，生长/分化因子-5 （GDF-5）转录本在细胞凝聚过程中被诱导，BMP-6转录本在软骨结节形成过程中被诱导，并随着分化的ATDC5细胞变得肥大而下降，BMP-7转录本仅在细胞钙化后才被检测到。外源添加BMP-4确实促进了早期分化。BMP-4和BMP-6也能刺激细胞的后期分化，因此，BMP信号的累积增加促进了细胞分化步骤的顺序转换。这些结果表明，内源性bmp的协调表达参与了ATDC5细胞软骨分化的进程。
Clonal cell line ATDC5 enables the monitoring of the early- and late-phase chondrogenic differentiation in a single culture, Undifferentiated ATDC5 cells differentiate into type ii collagen expressing chondrocytes through a cellular condensation stage (early-phase differentiation) and then to type X collagen-expressing hypertrophic chondrocytes (late-phase differentiation). progression of cellular differentiation was accelerated by the activation of bone morphognetic protein (BMP) signaling. ATDC5 cells expressed transcripts for at least four members of the BMP family. The BMP-I transcripts were expressed in all stages of differentiation, as were transcripts for BMP type 1A receptor (ALK-3) and BMP type II receptor. In contrast, transcripts for Growth/Differentiation factor-5 (GDF-5) were induced during a cellular condensation, and those for BMP-6 were induced during the formation of cartilage nodules, and declined as the differentiated ATDC5 cells became hypertrophic, and BMP-7 transcripts were only detected after cells became calcified. Exogenously added BMP-4 indeed promoted the early-phase differentiation. Late-phase differentiation of cells was also stimulated by BMP-4 and BMP-6, Thus, the cumulative increase in BMP signaling promoted the sequential transitions of differentiation steps of cells. These results indicate that the coordinated expressions of endogenous BMPs are involved in the progression of chondrogenic differentiation in ATDC5 cells.