Polystyrene nanoplastics mediated the toxicity of silver nanoparticles in zebrafish embryos
Polystyrene nanoplastics mediated the toxicity of silver nanoparticles in zebrafish embryos
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DOI：
10.3389/fmars.2023.1195125
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发表时间：
2023-06
期刊：
影响因子：
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通讯作者：
Zhenhua Yan;Yixin Zhou;Peiyuan Zhu;Xuhui Bao;Pengpeng Su
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文献类型：
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作者：
Zhenhua Yan;Yixin Zhou;Peiyuan Zhu;Xuhui Bao;Pengpeng Su
纳米塑料和纳米材料在水环境中的广泛分布令人极为关切。纳米塑料已被发现可以调节其他环境污染物对生物体的毒性，而很少有研究集中于它们对纳米材料的影响。因此，本研究从急性毒性、氧化应激、细胞凋亡、免疫毒性和代谢能力等方面评价了聚苯乙烯(PS)纳米塑料对纳米银(AgNPs)对斑马鱼(Danio Rerio)胚胎毒性的影响。结果表明，PS纳米塑料的存在可以作为水体中共存的AgNPs的载体。AgNPs中Ag+的释放率高达4.23%。共添加PS纳米塑料后，AgNPs对斑马鱼胚胎的致死效应没有明显改变。而PS纳米塑料显著增强了AgNPs对斑马鱼(SO1、CAT、MT2、MTF-1和Cox1)抗氧化和代谢相关基因表达的影响，同时降低了AgNPs诱导的细胞凋亡和免疫毒性(Caspase9、NFKβ、CEBP和IL-1β)。这表明PS纳米塑料的存在抑制了AgNPs对斑马鱼的遗传毒性。AgNPs释放出的Ag+可能与AgNPs对斑马鱼的毒性有关，而AgNPs随后在PS纳米塑料上的吸附和团聚以及释放的Ag+可能会减轻其毒性。
The widespread distribution of nanoplastics and nanomaterials in aquatic environments is of great concern. Nanoplastics have been found to modulate the toxicity of other environmental pollutants in organisms, while few studies have focused on their influences on nanomaterials. Thus, this study evaluated the influences of polystyrene (PS) nanoplastics on the toxicity of silver nanoparticles (AgNPs) to zebrafish (Danio rerio) embryos, including acute toxicity, oxidative stress, apoptosis, immunotoxicity, and metabolic capability. The results showed that the presence of PS nanoplastics could act as a carrier of the co-existing AgNPs in waters. The release ratio of Ag+ from AgNPs was up to 4.23%. The lethal effects of AgNPs on zebrafish embryos were not significantly changed by the co-added PS nanoplastics. Whereas, the alterations in gene expression related to antioxidant and metabolic capability in zebrafish (sod1, cat, mt2, mtf-1, and cox1) caused by AgNPs were significantly enhanced by the presence of PS nanoplastics, which simultaneously lowered the apoptosis and immunotoxicity (caspase9, nfkβ, cebp, and il-1β) induced by AgNPs. It suggests the presence of PS nanoplastics suppressed the AgNPs-induced genotoxicity in zebrafish. The released Ag+ from AgNPs may be responsible for the toxicity of AgNPs in zebrafish, while the subsequent absorption and agglomeration of AgNPs and the released Ag+ on PS nanoplastics may alleviate the toxicity.