The mitochondrial genome of the primary screwworm fly Cochliomyia hominivorax (Diptera: Calliphoridae)
The mitochondrial genome of the primary screwworm fly Cochliomyia hominivorax (Diptera: Calliphoridae)
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DOI：
10.1046/j.1365-2583.2000.00215.x
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发表时间：
2000-10-01
影响因子：
2.6
通讯作者：
Azeredo-Espin, AML
中科院分区：
文献类型：
--
作者：
Lessinger, AC;Junqueira, ACM;Azeredo-Espin, AML
测定了螺杆虫（Cochliomyia hominivorax）线粒体基因组全序列。该基因组的大小为16022 bp，与典型的短肢动物mtDNA相对应。描述了COI的丝氨酸起始密码子和COII、NADH 5和NADH 4基因的不完全终止密码子。C. hominivorax mtDNA的核苷酸组成富含77%的at，这反映在蛋白质编码基因中富含at的密码子占主导地位。非最优密码子使用在人源弓形虫线粒体基因中普遍存在。系统发育分析表明，该物种作为一个单系类群分布，并将C. hominivorax （Calyptratae）和Acalyptratae聚集在一个典型的短肢类群中。本文讨论了线粒体基因组诊断限制性位点的鉴定及其与以往RFLP分析的相关性。
The complete sequence of the mitochondrial genome of the screwworm Cochliomyia hominivorax was determined. This genome is 16 022 bp in size and corresponds to a typical Brachycera mtDNA. A Serine start codon for COI and incomplete termination codons for COII, NADH 5 and NADH 4 genes were described. The nucleotide composition of C. hominivorax mtDNA is 77% AT-rich, reflected in the predominance of AT-rich codons in protein-coding genes. Non-optimal codon usage was commonly observed in C. hominivorax mitochondrial genes. Phylogenetic analysis distributed the Acalypterate species as a monophyletic group and assembled the C. hominivorax (Calyptratae) and the Acalyptratae in a typical Brachycera cluster. The identification of diagnostic restriction sites on the sequenced mitochondrial genome and the correlation with previous RFLP analysis are discussed.