Micro-Raman spectroscopy used to identify and grade human skin pilomatrixoma
Micro-Raman spectroscopy used to identify and grade human skin pilomatrixoma
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DOI：
10.1002/jemt.20229
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发表时间：
2005-10-01
影响因子：
2.5
通讯作者：
Lin, SY
中科院分区：
文献类型：
--
作者：
Cheng, WT;Liu, MT;Lin, SY
应用拉曼显微光谱分析了人皮肤毛发基质瘤(PMX)肿块的结构形态和化学成分的变化。以正常真皮、I型胶原和羟基磷灰石(HA)为对照。其中1例为软性肿块，1例为硬性肿块，组织病理学检查显示有一定钙化沉积。正常皮肤真皮的拉曼光谱与I型胶原的拉曼光谱相似，证实了I型胶原是正常皮肤真皮的主要成分。正常皮肤真皮与软硬PMX肿块的拉曼峰强度在1,622~1,558、1,400~1,230、1,128、1,000~850、749和509 cm(-1)处有明显差异。特别是，在硬PMX质量中，对应于酰胺I带的1,665 cm(-1)峰移动到1,655 cm(-1)，对应于酰胺III带的1,246 cm-1峰的强度减弱。软质和硬质PMX的拉曼光谱中分别出现了960 cm(-1)的肩峰和960 cm(-1)的弱峰，说明在PMX组织中发生了HA的钙化，尤其是硬PMX质量块。结果表明，显微拉曼光谱可以为鉴别正常皮肤真皮以及不同PMX组织中真皮化学成分的差异提供一种高度灵敏和特异的方法。
Raman microspectroscopy was applied to analyze the changes in structural conformation and chemical composition of the mass of human skin pilomatrixoma (PMX). The normal skin dermis, collagen type 1, and hydroxyapatite (HA) were used as control. The excised specimens from two patients diagnosed as a typical PMX were detected, in which one specimen was a soft mass, but the other was a hard mass with somewhat calcified deposits via histopathological examination. The Raman spectrum of normal skin dermis was found to be similar to the Raman spectrum of collagen type 1, confirming that the collagen type I was a predominant component in normal skin dermis. The differences of Raman peak intensity between normal skin dermis and soft or hard PMX mass were obvious at 1,622-1,558, 1,400-1,230, 1,128, 1,000-850, 749, and 509 cm(-1). In particular, the peak at 1,665 cm(-1) assigned to amide I band shifted to 1,655 cm(-1) and the peak at 1,246 cm-1 corresponding to amide III band was reduced in its intensity in hard PMX mass. The significant changes in collagen content and its structural conformation, the higher content of tryptophan, and disulfide formation in PMX masses were markedly evidenced.In addition, the shoulder and weak peak at 960 cm(-1) assigned to the stretching vibration of PO43- of HA also appeared respectively in the Raman spectra of soft and hard PMX masses, suggesting the occurrence of calcification of HA in the PMX tissue, particularly in the hard PMX mass. The result indicates that the micro-Raman spectroscopy may provide a highly sensitive and specific method for identifying normal skin dermis and how it differs in chemical composition from different PMX tissues.