Ciliary functional analysis: Beating a path towards standardization
Ciliary functional analysis: Beating a path towards standardization
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DOI：
10.1002/ppul.24439
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发表时间：
2019-07-16
影响因子：
3.1
通讯作者：
Chilvers, Mark A.
中科院分区：
文献类型：
--
作者：
Kempeneers, Celine;Seaton, Claire;Chilvers, Mark A.
原发性纤毛运动障碍是一种遗传性疾病，其中呼吸纤毛是静止的，或以缓慢或运动障碍的方式跳动，导致粘膜纤毛清除受损和严重的鼻窦炎。一种诊断测试是使用数字高速视频显微镜（DHSV）的睫状体功能分析，其允许实时分析完整的睫状体功能，包括睫状体搏动频率（CBF）和睫状体搏动模式（CBP）。然而，DHSV缺乏标准化。在本文中，目前的知识DHSV睫状体功能分析，并建议睫状体样本的收集和处理的标准化协议。提出了一个定量和定性的CBP评价系统，用于制定国际共识协议，并确定了未来的DHSV研究领域的建议。
Primary ciliary dyskinesia is an inherited disorder in which respiratory cilia are stationary, or beat in a slow or dyskinetic manner, leading to impaired mucociliary clearance and significant sinopulmonary disease. One diagnostic test is ciliary functional analysis using digital high-speed video microscopy (DHSV), which allows real-time analysis of complete ciliary function, comprising ciliary beat frequency (CBF) and ciliary beat pattern (CBP). However, DHSV lacks standardization. In this paper, the current knowledge of DHSV ciliary functional analysis is presented, and recommendations given for a standardized protocol for ciliary sample collection and processing. A proposal is presented for a quantitative and qualitative CBP evaluation system, to be used to develop international consensus agreement, and future DHSV research areas are identified.