Zebrafish periostin is required for the adhesion of muscle fiber bundles to the myoseptum and for the differentiation of muscle fibers
Zebrafish periostin is required for the adhesion of muscle fiber bundles to the myoseptum and for the differentiation of muscle fibers
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DOI：
10.1016/j.ydbio.2003.12.007
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发表时间：
2004-03-15
影响因子：
2.7
通讯作者：
Kudo, A
中科院分区：
文献类型：
--
作者：
Kudo, H;Amizuka, N;Kudo, A
鱼类的肌隔膜由致密的胶原蛋白组成，是胚胎中形成的结缔组织层，将体节与躯干分开，其结构和功能与哺乳动物的肌腱相似。肌隔膜和肌腱都是将肌肉收缩力传递给骨骼和邻近肌肉的传递者，它们的结构对于脊椎动物的运动是不可或缺的。我们克隆了斑马鱼骨膜蛋白基因，并检查了其在肌隔膜中的表达和功能。胚胎中的表达起始于分节早期各分节体节的吻端部；因此，观察到同色异谱条纹。在分割结束时，表达区域转移到横向肌间隔和肌节-表皮边界，并且每个肌节被骨膜蛋白包围。使用多克隆抗体，我们发现骨膜素蛋白定位于横肌隔膜。一致地，periostin吗啉代反义寡核苷酸导致肌隔膜形成缺陷、肌纤维分化延迟以及肌原纤维和肌隔膜之间的连接紊乱。我们在这里证明了骨膜素是参与肌隔膜形成的第一个分子，并提出肌隔膜表面的骨膜素分泌是肌纤维束粘附到肌隔膜和肌纤维分化所必需的。 (C) 2004 Elsevier Inc. 保留所有权利。
The myoseptum of fishes, composed of dense collagen, is a connective tissue layer that forms in the embryo, dividing somites from the trunk, and its structure and function are similar to those of the mammalian tendon. Both the myoseptum and tendon serve as the transmitter of muscular contractility to bones and adjoining muscles, and their structure is indispensable for movement of vertebrate animals. We cloned the zebrafish periostin gene and examined its expression and function in the myoseptum. The expression in embryos started in the rostral part of each segmented somite in the early segmentation stage; and consequently, metameric stripes were observed. At the end of segmentation, the expression region shifted to the transverse myoseptum and the myotome-epidermis boundary, and each myotome was surrounded by periostin. Using a polyclonal antibody, we found that the periostin protein was localized to the transverse myoseptum. Consistently, periostin morpholino antisense oligonucleotide led to defects in myoseptum formation, a delay in the differentiation of myofibers, and disorder of connection between myofibrils and myoseptum. We demonstrated here that periostin is the first molecule involved in myoseptum formation and propose that periostin secretion on the surface of the myoseptum is required for the adhesion of muscle fiber bundles to the myoseptum and the differentiation of muscle fibers. (C) 2004 Elsevier Inc. All rights reserved.