A SPERM-SUPPLIED PRODUCT ESSENTIAL FOR INITIATION OF NORMAL EMBRYOGENESIS IN CAENORHABDITIS-ELEGANS IS ENCODED BY THE PATERNAL-EFFECT EMBRYONIC-LETHAL GENE, SPE-11
A SPERM-SUPPLIED PRODUCT ESSENTIAL FOR INITIATION OF NORMAL EMBRYOGENESIS IN CAENORHABDITIS-ELEGANS IS ENCODED BY THE PATERNAL-EFFECT EMBRYONIC-LETHAL GENE, SPE-11
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DOI：
10.1016/0012-1606(89)90138-3
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发表时间：
1989-11-01
影响因子：
2.7
通讯作者：
STROME, S
中科院分区：
文献类型：
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作者：
HILL, DP;SHAKES, DC;STROME, S
秀丽隐杆线虫中 spe-11 基因的功能缺失突变会导致父系效应胚胎致死表型：纯合 spe-11 突变雄性的精子与野生型卵母细胞受精会导致合子发育异常，而纯合 spe-11 雌雄同体的卵母细胞在与野生型精子受精时发育正常。由精子纯合 spe-11 蠕虫受精的胚胎无法完成减数分裂，并在蛋壳形成、有丝分裂纺锤体方向和胞质分裂方面表现出缺陷。遗传分析表明，spe-11 基因在精子发生完成之前表达，野生型基因座编码存在于精子中的产物，并直接或间接参与启动秀丽隐杆线虫胚胎中早期事件的正确程序。 spe-11+ 基因产物在早期胚胎发生中的这种个体发生作用将 spe-11 突变与果蝇中发现的两种父本效应突变（ms (3) K81 和 pal）区分开来，这两种突变主要影响染色体行为。对 spe-11 的分析为对秀丽隐杆线虫早期胚胎发生中精子的功能进行基因剖析迈出了第一步。
Loss-of-function mutations in the spe-11 gene in Caenorhabditis elegans result in a paternal-effect embryonic-lethal phenotype: fertilization of wild-type oocytes by sperm for homozygous spe-11 mutant males leads to abnormal zygotic development, whereas oocytes from homozygous spe-11 hermaphrodites when fertilized by wild-type sperm develop normally. Embryos fertilized by sperm homozygous spe-11 worms fail to complete meiosis and show defects in eggshell formation, mitotic spindle orientation, and cytokinesis. Genetic analysis suggests that the spe-11 gene is expressed before the completion of spermatogenesis and that the wild-type locus encodes a product that is present in sperm and participates, directly or indirectly, in initiating the correct program of early events in C. elegans embryos. Such an ontogenetic role of the spe-11+ gene product in early embryogenesis distinguishes spe-11 mutations from the two paternal-effect mutations identified in Drosophila, ms (3) K81 and pal, which primarily affect chromosome behavior. Analysis of spe-11 provides the first step toward genetic dissection of the functions of the sperm in early embryogenesis in C. elegans.