Activating transcription factor 3 (ATF3) induction by axotomy in sensory and motoneurons: A novel neuronal marker of nerve injury
Activating transcription factor 3 (ATF3) induction by axotomy in sensory and motoneurons: A novel neuronal marker of nerve injury
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DOI：
10.1006/mcne.1999.0814
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发表时间：
2000-02-01
影响因子：
3.5
通讯作者：
Noguchi, K
中科院分区：
文献类型：
--
作者：
Tsujino, H;Kondo, E;Noguchi, K
激活转录因子3(ATF3)是转录因子ATF/CREB家族中的一员，在多种应激组织中被诱导表达。ATF3通过以同源或异源二聚体的形式与Jun蛋白结合到DNA位点来调节转录。本研究旨在探讨轴索损伤后ATF3在背根节和脊髓神经元中的表达及调控。幼龄大鼠背根神经节和脊髓不表达ATF3。周围神经切断后，几乎所有切断轴索的DRG神经元和运动神经元都立即诱导出ATF3，诱导的时程依赖于损伤部位到细胞体的距离。免疫组织化学双标结果显示，轴突切断后，表达ATF3的DRG神经元群包括表达c-jun的神经元，并且在运动神经元中同时表达ATF3和c-jun。与c-jun相反，ATF3在脊髓背角神经元中不能跨突触诱导。我们的结论是，ATF3在神经损伤后的脊髓感觉和运动神经元中特异性地表达，应该被认为是神经系统中神经损伤的一个独特的神经元标志物。
Activating transcription factor 3 (ATF3), a member of ATF/CREB family of transcription factors, is induced in a variety of stressed tissue. ATF3 regulates transcription by binding to DNA sites as a homodimer or heterodimer with Jun proteins. The purpose of this study was to examine the expression and regulation of ATF3 after axonal injury in neurons in dorsal root ganglia (DRG) and spinal cord. In naive rats, ATF3 was not expressed in the DRG and spinal cord. Following the cut of peripheral nerve, ATF3 was immediately induced in virtually all DRG neurons and motoneurons that were axotomized, and the time course of induction was dependent on the distance between the injury site and the cell body. Double labeling using immunohistochemistry revealed that the population of DRG neurons expressing ATF3 included those expressing c-jun, and in motoneurons ATF3 and c-jun were concurrently expressed after axotomy. In contrast to c-jun, ATF3 was not induced transsynaptically in spinal dorsal horn neurons. We conclude that ATF3 is specifically induced in sensory and motoneurons in the spinal cord following nerve injury and should be regarded as an unique neuronal marker of nerve injury in the nervous system.