ENDOTHELIAL CAVEOLAE HAVE THE MOLECULAR-TRANSPORT MACHINERY FOR VESICLE BUDDING, DOCKING, AND FUSION INCLUDING VAMP, NSF, SNAP, ANNEXINS, AND GTPASES
ENDOTHELIAL CAVEOLAE HAVE THE MOLECULAR-TRANSPORT MACHINERY FOR VESICLE BUDDING, DOCKING, AND FUSION INCLUDING VAMP, NSF, SNAP, ANNEXINS, AND GTPASES
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DOI：
10.1074/jbc.270.24.14399
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发表时间：
1995-06-16
影响因子：
4.8
通讯作者：
OH, P
中科院分区：
文献类型：
--
作者：
SCHNITZER, JE;LIU, J;OH, P
通过离散囊泡载体的运输至少部分是因为最近发现识别囊泡形成、对接和融合的关键蛋白质介体而被良好地建立。在所有真核生物中，对N-乙基马来酰亚胺（NEM）敏感的一般机制是运输不同的囊泡载体所必需的。许多内皮细胞有大量的无包膜质膜囊泡或小窝，这已被报道具有相当大的争议，在运输功能。我们最近已经表明，与其它囊泡转运系统一样，小窝介导的内吞作用和胞吞转运作用被MEM抑制（Schnitzer，J.E.，阿拉德，J.，Oh，P.（1995）268，H48-H55）。在此，我们通过利用我们最近开发的从大鼠肺组织纯化内皮细胞小窝的方法（Schnitzer，J.E.，噢，皮，雅各布森，B。美国，和Dvorak，A. M.等人（1995）Proc. Acad. Sci.联合S. A. 92，1759-1763），以显示这些小窝含有已知介导囊泡形成、对接和/或融合的不同方面的关键蛋白，包括vSNARE VAMP-2、单体和三聚体GTP酶、膜联蛋白II和VI、以及NEM敏感性融合因子NSF沿着其附着蛋白SNAP。与神经元VAMP一样，这种内皮VAMP对肉毒杆菌B和破伤风神经毒素的裂解敏感。内皮细胞中的小窝确实像其他载体囊泡，并含有类似的NEM敏感的分子机制进行运输。
Transport by discrete vesicular carriers is well established at least in part because of recent discoveries identifying key protein mediators of vesicle formation, docking, and fusion. A general mechanism sensitive to N-ethylmaleimide (NEM) is required for the transport of a divergent group of vesicular carriers in all eukaryotes. Many endothelia have an abundant population of noncoated plasmalemmal vesicles or caveolae, which have been reported with considerable controversy to function in transport. We recently have shown that like other vesicular transport systems, caveolae-mediated endocytosis and transcytosis are inhibited by MEM (Schnitzer, J. E., Allard, J., and Oh, P. (1995) Am. J. Physiol. 268, H48-H55). Here, we continue this work by utilizing our recently developed method for purifying endothelial caveolae from rat lung tissue (Schnitzer, J. E., Oh, P., Jacobson, B. S., and Dvorak, A. M. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 1759-1763) to show that these caveolae contain key proteins known to mediate different aspects of vesicle formation, docking, and/or fusion including the vSNARE VAMP-2, monomeric and trimeric GTPases, annexins II and VI, and the NEM-sensitive fusion factor NSF along with its attachment protein SNAP. Like neuronal VAMPs, this endothelial VAMP is sensitive to cleavage by botulinum B and tetanus neurotoxins. Caveolae in endothelium are indeed like other carrier vesicles and contain similar NEM-sensitive molecular machinery for transport.