Functional analysis of the 5' flanking sequence in the ovine beta 1-adrenergic receptor gene.
Functional analysis of the 5' flanking sequence in the ovine beta 1-adrenergic receptor gene.
复制标题
绵羊 β1-肾上腺素能受体基因 5 侧翼序列的功能分析。
DOI：
10.1006/bbrc.1995.2507
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Padbury,JF
中科院分区：
文献类型：
--
作者：
Tseng,YT;Waschek,JA;Padbury,JF
Functional data for the promoter of the β1-adrenergic receptor (β1AR) gene are lacking. We previously cloned the ovine β1AR gene and mapped the transcription start sites. We now report data on ovine β1AR gene expression obtained by transient transfection. Progressive deletion of upstream 5′ flanking region moderately increased transcription activity in three cell lines compared to the full-length promoter. Deletion of sequences between −1530 and −953 produced the greatest increase in transcriptional activity. This region encompassed a putative GRE and an AP1 site. Deletion of the transcription start sites eliminated nearly all of the activity. Dexamethasone significantly increased activity of each of the promoter constructs tested in C6 glioma cells and an embryonic myocardial cell line, W1 cells. T3 alone had no effect and cotreatment with T3 did not augment the effects of dexamethasone. We conclude, basal transcription activity is repressed by a mechanism which operates through element(s) in the proximal promoter. Glucocorticoids increase transcription through mechanism(s) within the same region. We speculate that this region in the ovine β1AR promoter may be responsible for its unique transcription regulation.
