Stimulus-response coupling in mammalian ciliated cells. Demonstration of two mechanisms of control for cytosolic [Ca2+]
Stimulus-response coupling in mammalian ciliated cells. Demonstration of two mechanisms of control for cytosolic [Ca2+]
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哺乳动物纤毛细胞中的刺激-反应耦合。
DOI：
10.1016/s0006-3495(89)82772-9
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发表时间：
1989
影响因子：
3.4
通讯作者：
Verdugo,P
中科院分区：
文献类型：
--
作者：
Villalon,M;Hinds,TR;Verdugo,P
细胞内[Ca 2 +]的变化已被提出耦合纤毛运动的刺激，然而，与纤毛细胞的刺激相关的细胞内游离[Ca 2 +]的波动的定量测量尚未被研究。在兔输卵管纤毛细胞的原代培养物中，微摩尔浓度的三磷酸腺苷（ATP）和前列腺素F2 α（PGF 2 α）产生的纤毛活性的刺激与细胞内[Ca 2 +]的瞬时增加有关。钙通道阻断剂LaCl 3可抑制ATP引起的胞浆[Ca ~（2+）]_i和纤毛搏动频率的升高，但对PGF_（2 α）引起的胞浆[Ca ~（2+）]_i和纤毛搏动频率的升高无影响。这些结果是第一次直接证明，细胞内[Ca 2 +]的波动与哺乳动物上皮细胞中纤毛搏动频率增加有关。目前的研究结果表明，睫状体上皮刺激耦合的两种不同的钙依赖性机制：ATP通过嘌呤能受体作用，耦合到跨膜内流的Ca 2+，和PGF 2 α通过受体介导的细胞内螯合钙的释放。
Changes of cytosolic [Ca2+] have been proposed to couple stimulation of ciliary movement, however, quantitative measurements of fluctuations of intracellular free [Ca2+] associated with stimulation of ciliated cells have not been investigated. In primary cultures of rabbit oviductal ciliated cells, the stimulation of ciliary activity produced by micromolar concentrations of adenosine triphosphate (ATP) and prostaglandin F2 alpha (PGF2 alpha) was associated with a transient increase of intracellular [Ca2+]. Whereas the increase of cytosolic [Ca2+] and beat frequency produced by ATP were inhibited by the Ca-channel blocker LaCl3, the rise of cytosolic [Ca2+] and frequency of ciliary beat produced by PGF2 alpha was not affected by LaCl3. These results are the first direct demonstration that fluctuations of cytosolic [Ca2+] are associated with increased ciliary beat frequency in mammalian epithelial cells. The present findings suggest two different calcium-dependent mechanisms for stimulus-coupling in ciliary epithelium: ATP acting via purinergic receptor coupled to transmembrane influx of Ca2+, and PGF2 alpha acting via receptor-mediated release of intracellular sequestered Ca.