The effects of venous ensheathment on facial nerve repair in the rat.
The effects of venous ensheathment on facial nerve repair in the rat.
复制标题
静脉鞘套对大鼠面神经修复的影响。
DOI：
10.1002/lary.26501
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Hadlock,TessaA
中科院分区：
文献类型：
--
作者：
Chen,Pei;Knox,ChristopherJ;Yao,Linli;Li,Chunli;Hadlock,TessaA
目的探讨自体静脉鞘套术对大鼠面神经缝合后的保护作用，并检测鞘套术是否能促进神经修复后的功能恢复和轴突生长的准确性。正常对照组6只，面神经切断缝合组40只或横断和缝合修复，并附加静脉鞘套术（VE）。然后，大鼠进行功能测试，神经标本的组织学评估，或逆行tracing.ResultsAt术后第（POD）60天，静脉ensheathment显示周围组织没有粘连。两种手术方式（SR组和VE组）的神经瘤形成率差异无统计学意义（P< 0.05）。面神经手术后，面神经核运动神经元的逆行标记异常紊乱。在所有操作组中，在所有时间点的外周操作后均可观察到双逆行标记神经元，表明再生期间的异常轴突分支。结论自体静脉鞘套术不能促进面神经功能恢复，也不能提高轴突再生的准确性。进一步的研究是必要的，以阐明静脉鞘在其他运动和感觉神经模型中的作用。证据水平NA。喉镜，127：1558-1564，2017
ObjectiveTo investigate the protective effect of autologous venous ensheathment on sutured rat facial nerve and to test whether the ensheathment could improve the functional recovery of repaired nerve and accuracy of axonal growth.Study DesignIn vivo study.MethodsForty‐six rats were examined, with six rats serving as normal controls and 40 receiving facial nerve transection and suture repair (SR) or transection and suture repair with an additional venous ensheathment (VE). The rats were then subjected to functional testing, histological assessment of nerve specimens, or retrograde tracing, respectively.ResultsAt the postoperative day (POD) 60, the venous ensheathment showed no adhesion at the surrounding tissues. No significant difference in neuroma formation was found between the two surgical manipulations (SR and VE groups) (P< 0.05). Retrogradely labeled motoneurons in facial nuclei were extremely disorganized after the facial nerve undertook surgical manipulation. In all manipulated groups, double retrogradely labeled neurons, indicative of aberrant axonal branching during regeneration, could be observed after peripheral manipulation across all time points. With the two facial surgical manipulations, the average count of double‐labeled neurons at POD 60 was significantly less than at POD 21 (P< 0.05).ConclusionAutologous venous ensheathment could not help with the functional recovery of facial nerve or improve the accuracy of axonal regeneration. Further studies are warranted to elucidate the effects of venous ensheathment in other motor and sensory nerve models.Level of EvidenceNA.Laryngoscope, 127:1558–1564, 2017