Respiratory disease associated with bovine coronavirus infection in cattle herds in Southern Italy
Respiratory disease associated with bovine coronavirus infection in cattle herds in Southern Italy
复制标题
DOI：
10.1177/104063870802000105
复制
发表时间：
2008-01-01
影响因子：
1.5
通讯作者：
Buonavoglia, Canio
中科院分区：
文献类型：
--
作者：
Decaro, Nicola;Campolo, Marco;Buonavoglia, Canio
据报道，意大利牛群中爆发了四起与牛冠状病毒（BCoV）感染相关的牛呼吸道疾病（BRD）。在 3 次疫情中，仅在 2-3 个月大的饲养场犊牛中观察到 BRD，而在其余的疫情中，泌乳牛、小母牛和犊牛同时受到影响。通过使用逆转录聚合酶链反应 (RT-PCR)，在所有暴发疫情中均检测到 BCoV RNA，且没有证据表明存在并发病毒病原体（即牛呼吸道合胞病毒、牛疱疹病毒 1 型、牛病毒性腹泻病毒、牛副流感病毒）。仅从两次 BRD 爆发中发现了牛的常见细菌：葡萄球菌属。奇异变形杆菌（爆发 1）和溶血性曼海姆氏菌（爆发 4）。最近建立的实时 RT-PCR 测定表明，鼻分泌物中的病毒 RNA 载量范围为 3.10 x 10(2) 至 7.50 x 10(7) RNA 拷贝/pl 模板。除第一次疫情外，从所有疫情的呼吸道标本中分离出牛冠状病毒，其中实时 RT-PCR 在鼻拭子中发现病毒滴度非常低。
Four outbreaks of bovine respiratory disease (BRD) associated with bovine coronavirus (BCoV) infection in Italian cattle herds were reported. In 3 outbreaks, BRD was observed only in 2-3-month-old feedlot calves, whereas in the remaining outbreak, lactating cows, heifers, and calves were simultaneously affected. By using reverse transcription polymerase chain reaction (RT-PCR), BCoV RNA was detected in all outbreaks without evidence of concurrent viral pathogens (i.e., bovine respiratory syncytial virus, bovine herpesvirus type 1, bovine viral diarrhea virus, bovine parainfluenza virus). Common bacteria of cattle were recovered only from 2 outbreaks of BRD: Staphylococcus spp. and Proteus mirabilis (outbreak 1) and Mannheimia haemolytica (outbreak 4). A recently established real-time RT-PCR assay showed that viral RNA loads in nasal secretions ranged between 3.10 x 10(2) and 7.50 x 10(7) RNA copies/pl of template. Bovine coronavirus was isolated from respiratory specimens from all outbreaks except outbreak I, in which real-time RT-PCR found very low viral titers in nasal swabs.