Heart rate variability, a target for the effects of angiotensin II in the brain of the trout Oncorhynchus mykiss
Heart rate variability, a target for the effects of angiotensin II in the brain of the trout Oncorhynchus mykiss
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心率变异性是血管紧张素 II 在虹鳟大脑中作用的目标
DOI：
10.1016/s0006-8993(02)02903-7
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发表时间：
2002
期刊：
影响因子：
2.9
通讯作者：
J. Blanc
中科院分区：
文献类型：
--
作者：
J. Mével;N. Mimassi;F. Lancien;D. Mabin;J. Boucher;J. Blanc
这项研究是在未麻醉的虹鳟鱼上进行的，虹鳟鱼配备了两个ECG电极和一个脑室内（i. c. v.）微指南在三个30分钟的实验期间记录ECG信号，并在稳定的记录期间进行心率变异性（HRV）频谱分析。在对照期进行第一次记录，鳟鱼的平均心率（HR）为44±2 bpm。心电图R-R间期信号的总功率谱密度（PSD）为21233±4400 ms ~ 2/Hz。以0.16 Hz为中心的主要高频（HF）谱带和以0.04 Hz为中心的次要低频（LF）谱带是PSD的两个主要分量。在第二阶段期间i. c. v.注射0.5 μl溶剂对平均HR（43±2 bpm）、总PSD（24693±6394 ms 2/Hz）或两个主要光谱带的中心频率和功率均无显著统计学影响。相反，在第三次记录期间，i. c. v.注射ANGII（1.5，6.25和50 pmol）诱导平均HR显著增加（分别为+3%，+15%，+30%），但肽对总PSD的影响更明显，其显著降低（分别为-27%，-65%，-76%）。注射50 pmol ANGII后，PSD的两个主要光谱带完全钝化。在另一组对照鳟鱼中，腹膜内（i. p.）注射阿托品可消除ECG的R-R间期信号的PSD，表明副交感神经系统是鳟鱼HRV的主要贡献者。因此，我们的研究结果首次证明，至少在非哺乳动物物种中，i. c. v.注射天然ANGII显著降低HRV。我们假设，血管紧张素Ⅱ在鳟鱼的大脑改变了模式的电活动沿沿着节前心脏迷走神经运动神经元。
This study was conducted on unanesthetized rainbow trout equipped with two ECG electrodes and with an intracerebroventricular (i.c.v.) micro-guide. The ECG signal was recorded during three experimental sessions of 30 min and the heart rate variability (HRV) spectral analysis was performed during stabilized periods of recording. The first recording session was conducted during the control period and the mean heart rate (HR) of the trout was 44±2 bpm. The total power spectral density (PSD) of the R–R interval signal of the ECG was 21233±4400 ms2/Hz. A major high frequency (HF) spectral band centered at 0.16 Hz and a minor low frequency (LF) spectral band centered at 0.04 Hz were the two main components of the PSD. An i.c.v. injection of 0.5 μl of vehicle during the second session had no significant statistical effect, either on the mean HR (43±2 bpm), the total PSD (24693±6394 ms2/Hz) or on the center frequency and power of the two main spectral bands. Conversely, an i.c.v. injection of ANGII (1.5, 6.25 and 50 pmol) during the third recording session induced a significant increase in the mean HR (+3%, +15%, +30%, respectively) but the effect of the peptide was more obvious on the total PSD which was profoundly decreased (−27%, −65%, −76%, respectively). The two main spectral bands of the PSD were totally blunted after the injection of 50 pmol of ANGII. In another group of control trout, intraperitoneal (i.p.) injection of atropine abolished the PSD of the R–R interval signal of the ECG demonstrating that the parasympathetic system is the main contributor of HRV in trout. Our results have thus demonstrated for the first time, at least in a non-mammalian species, that i.c.v. injection of native ANGII profoundly reduces HRV. We hypothesize that ANGII in the brain of the trout alters the pattern of the electrical activity along preganglionic cardiac vagal motoneurons.
影响因子：
56.9
作者：
AKSELROD, S;GORDON, D;COHEN, RJ
通讯作者：
COHEN, RJ