The Role of the Notch Signal Pathway in Mucosal Cell Metaplasia in Mouse Acute Otitis Media.
The Role of the Notch Signal Pathway in Mucosal Cell Metaplasia in Mouse Acute Otitis Media.
复制标题
Notch信号通路在小鼠急性中耳炎粘膜细胞化生中的作用。
DOI：
10.1038/s41598-017-04639-z
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发表时间：
2017-07-04
影响因子：
4.6
通讯作者：
Ren DD
中科院分区：
文献类型：
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作者：
Liu X;Cong N;Cheng X;Ma R;Wang J;Huang YB;Zhao M;Wang XW;Chi FL;Ren DD
中耳炎(OM)是儿童和成人患者发病率的主要原因。这种炎症状态的特征是粘液细胞增生，被认为是中耳粘膜产生粘蛋白。我们感兴趣的是Notch信号通路在这一炎症过程中的作用。采用急性中耳炎(AOM)小鼠模型，通过中耳注射肺炎链球菌行组织病理学检查和定量RT-PCR检测急性炎症、杯状细胞增生和纤毛丢失情况，并检测Notch信号通路相关基因的表达。黏液细胞标志物Argr2和MUC5AC上调，纤毛细胞标志物Foxj1和Dnai2下调。此外，AOM中编码Notch受体和配体的基因(Notch1、Notch2、Notch3、Notch4和Dll1)以及Notch靶基因(Hes1、Hes5、Hey1、NRARP)显著减少。Notch1和Jagged1在小鼠中耳上皮细胞中的表达也呈下调趋势。综上所述，本研究提示Notch信号通路的下调参与了AOM时粘膜的增生。
Otitis media (OM) is a major cause of morbidity in pediatric and adult patients. This inflammatory condition is characterized by mucous cell hyperplasia that is thought to produce mucins from the middle ear mucosa. We are interested in the role of Notch signalling pathway in this inflammatory process. Using an acute otitis media (AOM) mouse model through injection of Streptococcus Pneumoniae into the middle ear, histopathologic examination and quantitative RT-PCR, acute inflammation with the thickness of mucosa, Goblet cell hyperplasia, and cilia loss were determined and gene expression related to the Notch signaling pathway were evaluated. Upregulation of the mucous cell markers, Argr2 and Muc5AC, and downregulation of the cilia cell marker, Foxj1 and Dnai2, were observed in AOM. In addition, genes encoding Notch receptors and ligands (Notch1, Notch2, Notch3, Notch4 and Dll1) and the Notch target genes (Hes1, Hes5, Hey1, NRARP) in AOM decreased significantly. The expression of the Notch1 and Jagged1 also showed down-regulation throughout the mouse middle ear epithelium. Taken together, this study suggests that downregulation of the Notch signaling pathway is involved in the mucosa hyperplasia during AOM.
DOI：
10.1177/000348940811700814
发表时间：
2008-08
期刊：
The Annals of otology, rhinology, and laryngology
影响因子：
--
作者：
Tsuchiya K;Komori M;Zheng QY;Ferrieri P;Lin J
通讯作者：
Lin J