MODULATION OF LEUKOCYTE MIGRATION IN MESENTERIC INTERSTITIUM
MODULATION OF LEUKOCYTE MIGRATION IN MESENTERIC INTERSTITIUM
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DOI：
10.1152/ajpheart.1994.267.4.h1573
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发表时间：
1994-10-01
影响因子：
4.8
通讯作者：
GRANGER, DN
中科院分区：
文献类型：
--
作者：
BIENVENU, K;HARRIS, N;GRANGER, DN
在大鼠肠系膜暴露于N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）、白三烯B-4（LTB（4））、血小板活化因子（PAF）或缺血再灌注（I-R）后，使用活体视频显微镜监测白细胞在肠系膜中的迁移。所有炎症刺激导致间质迁移率高于未受刺激的外渗白细胞。用FMLP刺激的细胞的中位迁移率显著高于用LTB（4）或PAF灌流期间或暴露于I-R后观察到的迁移率。PAF和LTB（4）受体拮抗剂处理后，I-R引起的白细胞迁移率增加并未减弱，表明这些脂质介质不是I-R诱导白细胞迁移的炎症介质。另外的实验表明，与FMLP刺激相关的白细胞迁移率没有显著改变，无论是嗜中性弹性蛋白酶和组织蛋白酶G的抑制剂，一种针对白细胞粘附糖蛋白CD 11/CD 18的单克隆抗体，或通过改变间质水化。这种体内模型提供了一种有用的新方法，用于定义调节血管外隔室内白细胞迁移的因素。
Intravital videomicroscopy was used to monitor the migration of leukocytes in rat mesenteric interstitium following exposure of the mesentery to either N-formylmethionyl-leucyl-phenylalanine (FMLP), leukotriene B-4 (LTB(4)), platelet-activating factor (PAF), or isehemia-reperfusion (I-R). All inflammatory stimuli resulted in interstitial migration rates that were higher than those measured in unstimulated extravasated leukocytes. The median migration rate of cells stimulated with FMLP was significantly higher than those observed during superfusion with either LTB(4) or PAF or following exposure to I-R. The enhanced leukocyte migration rates elicited by I-R were not attenuated by treatment with PAF- and LTB(4)-receptor antagonists, suggesting that these lipid mediators are not the inflammatory mediators responsible for I-R-induced leukocyte migration. Additional experiments revealed that the rate of leukocyte migration associated with FMLP stimulation was not significantly altered by either inhibitors of neutrophilic elastase and cathepsin G, a monoclonal antibody directed against the leukocyte adhesion glycoprotein CD11/CD18, or by altering interstitial hydration. This in vivo model provides a useful new approach for defining the factors that modulate leukocyte migration within the extravascular compartment.