Analysis of carotenogenic gene expression in petals and leaves of carnation (Dianthus caryophyllus L.)
Analysis of carotenogenic gene expression in petals and leaves of carnation (Dianthus caryophyllus L.)
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DOI：
10.1111/pbr.12061
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发表时间：
2013-08-01
期刊：
影响因子：
2
通讯作者：
Yagi, Masafumi
中科院分区：
文献类型：
--
作者：
Ohmiya, Akemi;Tanase, Koji;Yagi, Masafumi
康乃馨（Dianthus caryophyllus L.）属于石竹目石竹科。这一顺序的植物在花瓣中不积累类胡萝卜素。为了了解类胡萝卜素的积累是如何控制在康乃馨花瓣，我们分析了与类胡萝卜素积累相关的基因的表达。花瓣在发育的早期积累了少量的类胡萝卜素。随着花瓣成熟，它们的类胡萝卜素含量下降到极低的水平。相比之下，康乃馨叶含有大量的类胡萝卜素，是光合作用所必需的。大多数的胡萝卜素基因在花瓣中的表达水平类似于那些在叶片中，这些基因的表达水平增加花瓣发育过程中。编码八氢番茄红素合成酶和番茄红素环化酶的基因是例外。它们在花瓣中的表达量比在叶片中的表达量低。编码类胡萝卜素裂解双加氧酶4的基因在叶片和花瓣中都没有表达。这些数据表明，康乃馨花瓣中类胡萝卜素的低水平不是由酶降解，而是由类胡萝卜素生物合成的低速率引起的。
Carnation (Dianthus caryophyllus L.) belongs to the family Caryophyllaceae in the order Caryophyllales. Plants in this order do not accumulate carotenoids in petals. To understand how carotenoid accumulation is controlled in carnation petals, we analysed the expression of genes related to carotenoid accumulation. Petals at an early stage of development accumulated small amounts of carotenoids. As petals matured, their carotenoid content decreased to extremely low levels. In contrast, carnation leaves contained substantial amounts of carotenoids that are essential for photosynthesis. Most of the carotenogenic genes were expressed in petals at levels similar to those in leaves, and the expression levels of these genes increased during petal development. Genes encoding phytoene synthase and lycopene e-cyclase were exceptions. Their expression levels in petals were very low compared with those in leaves. Expression of the gene encoding carotenoid cleavage dioxygenase 4 was detected in neither leaves nor petals. These data suggest that the low levels of carotenoids in carnation petals are caused not by enzymatic degradation but rather by low rates of carotenoid biosynthesis.