Electropolymerized Receptor Coatings for the Quantitative Detection of Histamine with a Catheter-Based, Diagnostic Sensor
Electropolymerized Receptor Coatings for the Quantitative Detection of Histamine with a Catheter-Based, Diagnostic Sensor
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用于使用基于导管的诊断传感器定量检测组胺的电聚合受体涂层
DOI：
10.1021/acssensors.0c01844
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发表时间：
2020
期刊：
影响因子：
8.9
通讯作者：
Wackers G
中科院分区：
文献类型：
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作者：
Wackers G
在这篇文章中，我们报道了一种基于导管的仿生传感器的发展，作为功能性肠疾病微创诊断仪器的一步。组胺是小肠过敏和炎症过程的关键介质；然而，确定十二指肠黏膜的组胺水平是一个挑战，经典的生物受体不适合用于肠液的消化介质。因此，我们开发了用于阻抗传感电极的分子印迹聚吡咯涂层，可以在25 nM至1 μM的宽浓度范围内对未稀释的人体肠液中的组胺进行定量。电极对组氨酸作为竞争分子的交叉敏感性可以忽略不计，为了进行比较，我们还评估了非印迹和牛磺酸印迹聚吡咯对组胺的反应。此外，利用等效电路模型，我们发现聚吡咯对组胺的分子识别主要增加了电极-液体界面的电阻成分，而电容效应可以忽略不计。该传感器集成到导管中，测量差异以纠正肠道液体复杂基质中的非特异性吸附效应，单一触发频率足以确定组胺浓度。总之，这些特性有利于实时诊断测试。
In this article, we report on the development of a catheter-based, biomimetic sensor as a step toward a minimally invasive diagnostic instrument in the context of functional bowel disorders. Histamine is a key mediator in allergic and inflammatory processes in the small intestines; however, it is a challenge to determine histamine levels at the duodenal mucosa, and classical bioreceptors are unsuitable for use in the digestive medium of bowel fluid. Therefore, we have developed molecularly imprinted polypyrrole coatings for impedimetric sensing electrodes, which enable the quantification of histamine in nondiluted, human bowel fluid in a broad concentration range from 25 nM to 1 μM. The electrodes show negligible cross-sensitivity to histidine as a competitor molecule and, for comparison, we also evaluated the response of nonimprinted and taurine-imprinted polypyrrole to histamine. Furthermore, using equivalent-circuit modeling, we found that the molecular recognition of histamine by polypyrrole primarily increases the resistive component of the electrode–liquid interface while capacitive effects are negligible. The sensor, integrated into a catheter, measures differentially to correct for nonspecific adsorption effects in the complex matrix of bowel fluids, and a single triggering frequency is sufficient to determine histamine concentrations. Together, these features are beneficial for real-time diagnostic tests.