Urinary miRNA-377 and miRNA-216a as biomarkers of nephropathy and subclinical atherosclerotic risk in pediatric patients with type 1 diabetes
Urinary miRNA-377 and miRNA-216a as biomarkers of nephropathy and subclinical atherosclerotic risk in pediatric patients with type 1 diabetes
复制标题
DOI：
10.1016/j.jdiacomp.2017.10.014
复制
发表时间：
2018-02-01
影响因子：
3
通讯作者：
Mohammed, Hossam Hassan
中科院分区：
文献类型：
--
作者：
El-Samahy, Mona Husein;Adly, Amira AbdelMoneam;Mohammed, Hossam Hassan
背景：尿微rna （miRNAs）在慢性肾脏疾病（CKD）的发病机制中发挥作用。目的：检测50例1型糖尿病（T1DM）儿童和青少年尿中miR-377和miR-216a的表达，并与50例健康对照进行比较，评估其与蛋白尿程度、血糖控制程度以及作为动脉粥样硬化指标的颈动脉内膜厚度（CIMT）的关系。方法：根据尿白蛋白肌酐比值（UACR）将糖尿病受试者分为正常蛋白尿组和微量蛋白尿组。采用实时聚合酶链反应评估尿液mirna。采用高分辨率颈动脉超声测量CIMT。结果：尿miR-377在微量白蛋白尿患者中的表达（中位数，3.8）明显高于正常白蛋白尿患者和健康对照组的表达（中位数，3.8），分别为2.65和0.98
Background: Urinary microRNAs (miRNAs) play a role in the pathogenesis of chronic kidney disease (CKD).Aim: To identify the expression of urinary miR-377 and miR-216a in 50 children and adolescents with type 1 diabetes (T1DM) compared with 50 healthy controls and assess their relation to the degree of albuminuria, glycemic control and carotid intimal thickness (CIMT) as an index of atherosclerosis.Methods: Diabetic subjects were divided into normoalbuminuric and microalbuminuric groups according to urinary albumin creatinine ration (UACR). Urinary miRNAs were assessed using real time polymerase chain reaction. CIMT was measured using high resolution carotid ultrasound.Results: The expression of urinary miR-377 was significantly higher in patients with microalbumiuria (median, 3.8) compared with 2.65 and 0.98 in normoalbuminic patients and healthy controls, respectively (p