Bacteria transport in a porous medium: Retention of bacillus and pseudomonas on silica surfaces
Bacteria transport in a porous medium: Retention of bacillus and pseudomonas on silica surfaces
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多孔介质中的细菌传输：芽孢杆菌和假单胞菌在二氧化硅表面的保留
DOI：
10.1016/0043-1354(93)90216-5
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发表时间：
1993
期刊：
影响因子：
12.8
通讯作者：
C. Gerba
中科院分区：
文献类型：
--
作者：
T. Kinoshita;R. Bales;M. T. Yahya;C. Gerba
研究了流经填充有 0.5 毫米二氧化硅珠的实验室柱的水中荧光假单胞菌和枯草芽孢杆菌的运输情况。在约一孔体积的含细菌溶液通过柱后，细菌附着到二氧化硅的速率显示达到稳定状态。柱出口浓度为入口浓度的 6-80%。 P的少了。 pH 5.5 与 7.0 时的荧光延迟；由于两种细菌的 pHieps（等电点）都处于较低的 pH 值，因此这种差异表明，在该 pH 范围内静电斥力的变化对于确定多孔介质中细菌的保留相对不重要。这两个物种的运输都高度迟缓，表明它们都不能在沙质土壤中快速长距离运输。使用稳态传输模型充分描述了结果，其中细菌附着在土壤上作为伪一级过程。使用稳态胶体过滤模型计算得出的粘附效率 (α) 接近 1。切换到无细菌入口溶液后，在几个孔体积的柱流出物中继续出现细菌，表明附着是可逆的，尽管可逆性缓慢。
Pseudomonas fluorescensandBacillus subtilistransport was studied in water flowing through laboratory columns packed with 0.5-mm silica beads. The rate of bacterial attachment to the silica was shown to reach steady state after about one pore volume of bacteria-containing solution had passed through the column. Column-outlet concentrations were 6–80% of inlet concentrations. There was lessP. fluorescensretardation at pH 5.5 versus 7.0; since the pHieps (isoelectric point) for both bacteria are at lower pHs, this difference indicates that the change in electrostatic repulsion over this pH range was relatively unimportant in determining bacteria retention in the porous medium. Transport of both species was highly retarded, suggesting that neither could be quickly transported over long distances in sandy soils. Results were adequately described using a steady-state transport model with bacterial attachment to soil as a pseudo-first-order process. Sticking efficiency (α), calculated using the steady-state colloid-filtration model, was near one. The continued appearance of bacteria in the column effluent for several pore volumes after switching to a bacteria-free inlet solution shows attachment to be reversible, though slowly reversible.