A three-dimensional model of the mouse at embryonic day 9
A three-dimensional model of the mouse at embryonic day 9
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DOI：
10.1006/dbio.1999.9500
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发表时间：
1999-12-15
影响因子：
2.7
通讯作者：
Baldock, RA
中科院分区：
文献类型：
--
作者：
Brune, RM;Bard, JBL;Baldock, RA
本文描述了E9小鼠胚胎的数字三维模型。该模型是通过从连续的组织切片的图像重建数字扭曲，以消除失真，并具有约9 μ m的分辨率。该模型可以在任何平面进行数字切除，以提供类似于传统组织切片的图像。主要组织已被确定和划定的数字绘画，使解剖成分可以可视化和操纵的3-D表面和体积渲染的意见。这提供了解剖学的三维定义，这将为解释和理解小鼠胚胎中的空间数据提供有用的工具。模型的解剖结构进行了讨论，它提供了地标的解释和导航，或者它是意想不到的，在现有的描述E9小鼠胚胎。完整的解剖结构没有在本文中介绍，但将在CD-ROM中提供。模型构建的技术方面的详细说明包括在附录中。该模型是一系列模型中的第一个，这些模型将成为小鼠发育图谱/数据库的基础。该重建及其相关的解剖结构可以通过http://genex.hgu.mrc.ac.uk/的一些支持软件以各种数据格式提供。(C)北京：科学出版社.
This paper describes a digital, three-dimensional model of the mouse embryo at E9. The model was made by reconstruction from images of serial histological sections digitally warped to remove distortions and has a resolution of approximately 9 mu m. The model can be digitally resectioned in any plane to provide images which resemble conventional histological sections. The main tissues have been identified and delineated by digital painting so that the anatomical components can be visualized and manipulated in 3-D surface- and volume-rendered views. This provides a three-dimensional definition of anatomy that will provide a useful tool for interpreting and understanding spatial data in mouse embryos. The anatomy of the model is discussed where it provides landmarks for interpretation and navigation or where it is unexpected in light of existing descriptions of the E9 mouse embryo. The complete anatomy is not presented in this paper but will be available on CD-ROM. A detailed description of the technical aspects of the construction of the model is included in an appendix. The model is the first of a series that will form the basis for an atlas/database of mouse development. This reconstruction and its associated anatomy are available in a variety of data formats with some supporting software from http://genex.hgu.mrc.ac.uk/. (C) 1999 Academic Press.