THE DEVELOPMENT OF RAPID DIFFERENTIAL DIAGNOSIS TECHNIQUE FOR AVIAN TUMORS
THE DEVELOPMENT OF RAPID DIFFERENTIAL DIAGNOSIS TECHNIQUE FOR AVIAN TUMORS
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发表时间：
2002
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通讯作者：
W. Ping
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作者：
W. Ping
马立克氏病（MD）、网状内皮组织增生症（RE）和禽白血病（AL）是鸡最常见的肿瘤。本研究建立了一种基于PCR技术的鸡肿瘤快速鉴别诊断方法，该方法扩增了MDV的132 bp pr、ALV和REV的LTR基因片段。探讨了样品DNA提取方法的改进、PCR反应条件和参数的优化。此外，还评估了PCR的特异性和敏感性。整个过程从采样到最后得出结论，在一天的工作时间内完成，在我们的协议。结果表明，该PCR方法特异、灵敏、快速，为禽肿瘤快速鉴别诊断试剂盒的研制提供了可能。
Marek′s disease (MD), reticuloendotheliosis (RE) and avian leukosis (AL) are the most common tumors in chickens. A rapid differential diagnosis for avian tumors, which based on PCR technique was developed to amplify the specific gene fragments: 132bpr of MDV, LTRs of ALV and REV viral genomes. Improved protocol for sample DNA extraction, optimized condition and parameters for PCRs were approached. In addition, the specificity and the sensitivity of the PCRs were also evaluated. The whole procedure, from sampling to the final conclusion, was finished within one day′s workhour (8 h) in our protocol. The results demonstrated that the PCR protocol is a specific, sensitive and rapid tool for the differential diagnosis of avian tumors, which provides a possibility for the development of rapid differential detection kit for avian tumors.