Constitutive expression of mustard annexin, AnnBj1 enhances abiotic stress tolerance and fiber quality in cotton under stress
Constitutive expression of mustard annexin, AnnBj1 enhances abiotic stress tolerance and fiber quality in cotton under stress
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DOI：
10.1007/s11103-010-9615-6
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发表时间：
2010-06-01
影响因子：
5.1
通讯作者：
Kirti, P. B.
中科院分区：
文献类型：
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作者：
Divya, Kesanakurti;Jami, S. K.;Kirti, P. B.
膜联蛋白属于钙离子依赖性、磷脂和细胞骨架结合蛋白的多基因家族。它们已被证明在各种应激条件下上调。我们产生了转基因棉花植物表达芥菜膜联蛋白（AnnBj 1），这表现出对不同的非生物胁迫处理，如氯化钠，甘露醇，聚乙二醇和过氧化氢的耐受性增强。对这些处理的耐受性与转基因植株中过氧化氢水平降低和总过氧化物酶活性增强以及植物保护剂脯氨酸和蔗糖含量增加有关。他们表现出较高的保留总叶绿素和减少TBARS在叶盘测定与应力处理，并减少过氧化氢积累在气孔保卫细胞相比，野生型对应。在胁迫条件下，鲜重、相对含水量、干重均显著增加。氯化钠处理导致叶片中的匕首-吡咯啉-5-羧化酶合成酶基因的表达增强，转基因植物的叶片和纤维中的蔗糖磷酸合成酶、蔗糖合成酶和纤维素合成酶A基因的表达增强。转基因植株在逆境下保持了正常的种子发育、纤维品质和纤维素含量。
Annexins belong to a multigene family of Ca2+ dependent, phospholipid and cytoskeleton binding proteins. They have been shown to be upregulated under various stress conditions. We generated transgenic cotton plants expressing mustard annexin (AnnBj1), which showed enhanced tolerance towards different abiotic stress treatments like sodium chloride, mannitol, polyethylene glycol and hydrogen peroxide. The tolerance to these treatments was associated with decreased hydrogen peroxide levels and enhanced total peroxidase activity, enhanced content of osmoprotectants- proline and sucrose in transgenic plants. They showed higher retention of total chlorophyll and reduced TBARS in leaf disc assays with stress treatments, and decreased hydrogen peroxide accumulation in the stomatal guard cells when compared to their wild type counterparts. They also showed significantly enhanced fresh weight, relative water content, dry weight under stress. Treatment with sodium chloride resulted in enhanced expression of genes for a dagger-pyrroline-5-carboxylase synthetase in leaves, and sucrose phosphate synthase, sucrose synthase and cellulose synthase A in the leaves and fibers of transgenic plants. The transgenic plants maintained normal seed development, fiber quality and cellulose content under stress.