PHYSICAL-PROPERTIES OF SINGLE PHOSPHOLIPID-BILAYERS ADSORBED TO MICRO GLASS-BEADS - A NEW VESICULAR MODEL SYSTEM STUDIED BY H-2-NUCLEAR MAGNETIC-RESONANCE
PHYSICAL-PROPERTIES OF SINGLE PHOSPHOLIPID-BILAYERS ADSORBED TO MICRO GLASS-BEADS - A NEW VESICULAR MODEL SYSTEM STUDIED BY H-2-NUCLEAR MAGNETIC-RESONANCE
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DOI：
10.1016/s0006-3495(90)82382-1
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发表时间：
1990-08-01
影响因子：
3.4
通讯作者：
BLOOM, M
中科院分区：
文献类型：
--
作者：
BAYERL, TM;BLOOM, M
球形支持囊泡 (SSV) 是一种新模型系统，由吸附在尺寸分布较窄的球形玻璃珠上的单个二肉豆蔻酰磷脂酰胆碱 (DMPC) 双层组成，制备了两种不同尺寸（0.5 和 1.5 μm），并通过氘核磁共振 (2H-NMR) 研究了它们的物理性质。此类SSV样品可以制备成0.3至10μm之间的任何所需尺寸。 2H-NMR测量提供了在0.5至1.5μm直径范围内，频谱和横向弛豫时间对SSV曲率的强烈依赖性的证据。对于较大的 SSV（直径 1.5 μm），其粉末光谱和计算的定向光谱与 DMPC-d54 多层分散体获得的光谱相似。较小的 SSV 的线形表现出多层样品中未发现的温度依赖性。 SSV 在液晶相中保持稳定数天，但在凝胶状态下不可逆地转变为多层囊泡。通过1 H-NMR估计单个双层和玻璃珠表面之间的水层的平均厚度为17±。 5.ANG。
Spherical supported vesicles (SSVs), a new model system consisting of single dimyristoyl phosphatidylcholine (DMPC) bilayers adsorbed to spherical glass beads with a narrow size distribution, were prepared at two different sizes (0.5 and 1.5 .mu.m) and their physical properties were studied by deuterium nuclear magnetic resonance (2H-NMR). Such SSV samples can be prepared at any desired size between 0.3 and 10 .mu.m. The 2H-NMR measurements provide evidence for a strong dependence of the sepctra and the transverse relaxation times on the curvature of the SSVs in a diameter range between 0.5 and 1.5 .mu.m. For larger SSVs (1.5 .mu.m diameter) their powder spectra and their calculated oriented spectra are similar to those obtained for multilamellar dispersions of DMPC-d54. The lineshape of the smaller SSVs exhibits a temperature dependence which is not found in multilamellar samples. The SSVs are stable in the liquid crystalline phase over days but irreversibly change to multilamellar vesicles in the gel state. The average thickness of the water layer between the single bilayer and the glass bead surface was estimated by 1H-NMR to be 17 .+-. 5 .ANG.