Cloning and transcriptional expression of a leucokinin-like peptide receptor from the Southern cattle tick, Boophilus microplus (Acari: Ixodidae)
Cloning and transcriptional expression of a leucokinin-like peptide receptor from the Southern cattle tick, Boophilus microplus (Acari: Ixodidae)
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DOI：
10.1046/j.1365-2583.2000.00208.x
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发表时间：
2000-10-01
影响因子：
2.6
通讯作者：
Pietrantonio, PV
中科院分区：
文献类型：
--
作者：
Holmes, SP;He, H;Pietrantonio, PV
白细胞激肽是一种无脊椎动物神经肽，具有肌生长和利尿活性。只有一种白细胞激肽样肽受体是已知的，即来自软体动物滞水蛙的白细胞激肽受体。从南方牛蜱（Boophilus microplus）中克隆了一个编码白细胞激肽样肽受体的cDNA。这是已知的第一种来自阿卡里的神经肽受体，也是白细胞激肽样肽G蛋白偶联受体亚家族中已知的第二种。推导出的氨基酸序列显示出40%的同一性，血管紧张素受体。通过半定量逆转录聚合酶链反应测定，受体转录本存在于蜱的所有生命阶段。我们还建议，序列AAF 50775.1从果蝇基因组（CG10626）编码的第一个确定的昆虫白细胞激肽受体。
Leucokinins are invertebrate neuropeptides that exhibit myotropic and diuretic activity. Only one leucokinin-like peptide receptor is known, the lymnokinin receptor from the mollusc Lymnaea stagnalis. A cDNA encoding a leucokinin-like peptide receptor was cloned from the Southern cattle tick, Boophilus microplus, a pest of cattle world-wide. This is the first neuropeptide receptor known from the Acari and the second known in the subfamily of leucokinin-like peptide G-protein-coupled receptors. The deduced amino acid sequence exhibits 40% identity to the lymnokinin receptor. The receptor transcript is present in all tick life stages as determined by semiquantitative reverse transcription polymerase chain reaction. We also propose that the sequence AAF50775.1 from the Drosophila melanogaster genome (CG10626) encodes the first identified insect leucokinin receptor.