Influence of islet and bone marrow transplantation on the diabetes and immunodeficiency of BB rats.
Influence of islet and bone marrow transplantation on the diabetes and immunodeficiency of BB rats.
复制标题
胰岛和骨髓移植对BB大鼠糖尿病和免疫缺陷的影响。
DOI：
10.1016/s0026-0495(83)80013-4
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Barker,CF
中科院分区：
文献类型：
--
作者：
Naji,A;Silvers,WK;Kimura,H;Anderson,AO;Barker,CF
研究BB率自发性自身免疫性糖尿病患者胰岛移植的结果，以确定该疾病过程是否会损害移植的胰岛组织。由于BB大鼠基因不均匀，因此不能使用同基因移植物；因此，新生儿接种骨髓细胞使BB大鼠对WF移植抗原产生免疫耐受，可防止同种异体移植排斥反应。尽管由此产生的耐受性状态使得WF皮肤同种异体移植成功，但移植的胰岛在被免疫性胰岛素炎破坏之前，只能短暂地改善BB大鼠的自发性糖尿病。来自糖尿病种群的BB大鼠被发现患有T淋巴细胞及其亚群异常以及免疫反应模式缺陷。用大鼠淋巴细胞标记物特异性单克隆抗体分析，发现糖尿病大鼠的淋巴细胞减少。辅助性T细胞减少，抑制细胞/细胞毒亚群出现更严重的缺陷。研究发现，新生儿接种正常供体骨髓的BB大鼠患糖尿病的几率显著降低。此外，未接种BB大鼠中存在的T细胞功能、数量和微环境缺陷在骨髓接种BB大鼠中得到恢复，这可能与糖尿病发病率降低有关。
The results of islet transplantation in spontaneous autoimmune diabetes of BB rate were studied to determine whether this disease process might damage the transplanted islet tissue. Since BB rats are not genetically uniform, syngenetic grafts could not be used; therefore, allograft rejection was provented by rendering BB rats immunologically tolerant of WF transplantation antigens by neonatal inoculation with bone marrow cells. Despite the resultant tolerant stato, which permitted successful engraftment of WF skin allografts, the transplanted islets amellorated the spontaneous diabetes of BB rats only briafly before they were destroyed by immune insulitis. BB rats from the diabetic stock were found to suffer from abnormalities in T lymphocytes and their subsets as well as defective immune response patterns. When analyzed with monoclonal antibodies specific for rat lymphocyte markers, BB rats of the diabetic stock were found to be lymphocytopenic. There was a reduction in heiper T cells and a more severe deficit in the suppressor/cytotoxia subset. BB rats that were inoculated neonatally with bone marrow from normal donors were found to have a strikingly reduced incidence of diabetes. Moreover, the T cell functional, numerical, and microenvironmental defects that were present in noninoculated BB rats were restored in marrow-inoculated BB rats, findings possibly related to the decreased incidence of diabetes.