Effect of Cl- and H+ on the oxygen binding properties of glutaraldehyde-polymerized bovine hemoglobin-based blood substitutes
Effect of Cl- and H+ on the oxygen binding properties of glutaraldehyde-polymerized bovine hemoglobin-based blood substitutes
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DOI：
10.1021/bp049872l
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发表时间：
2004-09-01
影响因子：
2.9
通讯作者：
Palmer, AF
中科院分区：
文献类型：
--
作者：
Eike, JH;Palmer, AF
牛血红蛋白与戊二醛交联，产生不同分子量分布的高氧亲和性聚合血红蛋白分散体。设计了高氧亲和力的脱细胞氧载体，以便表现出更接近人红细胞（RBC）的氧释放曲线，而不表现出低氧亲和力的脱细胞氧载体所发生的固有增加的血管活性（1，2）。在不同pH值（7.0、7.4和8.0）和氯离子浓度下测量聚合血红蛋白分散体的氧解离曲线。未修饰的血红蛋白显示氧亲和力随着氯离子浓度的增加而增加，氧亲和力随着pH值的增加而降低，如先前在文献中所证明的那样（3）。对于戊二醛解聚的血红蛋白分散体，氧亲和力响应玻尔H+和Cl-浓度变化的能力减弱。然而，在酸性生理pH值（pH = 7），玻尔效应仍然存在于高浓度的Cl-。因此，玻尔效应对Cl-浓度有一定的依赖性。
Bovine hemoglobin was cross-linked with glutaraldehyde, resulting in high oxygen affinity polymeric hemoglobin dispersions of varying molecular weight distributions. High oxygen affinity acellular oxygen carriers were designed in order to exhibit oxygen release profiles closer to that of human red blood cells (RBCs), without exhibiting the inherent increased vasoactivity that occurs with low oxygen affinity acellular oxygen carriers (1, 2). Oxygen dissociation curves were measured for polymerized hemoglobin dispersions at various pH values (7.0, 7.4, and 8.0) and chloride ion concentrations. Unmodified hemoglobin showed an increase in oxygen affinity with increased chloride ion concentration and a decrease in oxygen affinity with increased pH, as was previously demonstrated in the literature (3). For glutaraldehyde-polymerized hemoglobin dispersions, the ability of the oxygen affinity to respond to changes in Bohr H+ and Cl- concentration was weakened. However, at acidic physiological pH (pH = 7), the Bohr effect was still present at high Cl- concentrations. Thus, the Bohr effect maintained some dependency on the Cl- concentration.