A Phenylurea Cytokinin, CPPU, Elevated Reducing Sugar and Correlated to Andrographolide Contents in Leaves of Andrographis paniculata (Burm. F.) Wall. Ex Nees
A Phenylurea Cytokinin, CPPU, Elevated Reducing Sugar and Correlated to Andrographolide Contents in Leaves of Andrographis paniculata (Burm. F.) Wall. Ex Nees
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苯脲细胞分裂素、CPPU、高还原糖以及与穿心莲叶中穿心莲内酯含量的相关性。
DOI：
10.1007/s12010-016-2238-x
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发表时间：
2017
影响因子：
3
通讯作者：
K. Supaibulwatana
中科院分区：
文献类型：
--
作者：
Phapawee Worakan;Netiya Karaket;Nuchada Maneejantra;K. Supaibulwatana
细胞分裂素是一种植物激素，在植物生长发育中起着多种作用。在本研究中，药用植物穿心莲的叶生物量和穿心莲内酯类化合物的产量在用合成细胞分裂素细胞分裂素-1-（2-氯-4-吡啶基）-3-苯基脲（CPPU）以0（水）、5或10 mg L−1外源处理后显著增加，并观察了处理24 h、48 h和7 d的结果。结果发现，CPPU可显着促进新腋芽的形成，并进一步促进分枝，使其比对照高4.6-5.6倍，从而使鲜重（FW）和干重（DW）增加。5 mg L−1的CPPU显著促进了叶片中总还原糖含量的最高2.5倍和根中的最高1.5倍。虽然CPPU处理显著影响叶绿素和类胡萝卜素含量的增加（1.2-1.6倍），但10 mg L−1的CPPU轻微导致叶片胁迫和叶绿素减少。有趣的是，5 mg L−1 CPPU可以提高穿心莲中的穿心莲内酯含量，穿心莲内酯是穿心莲中的一种抗感染活性化合物（比对照高2.2倍），在处理后24 h达到最高含量。本研究表明，CPPU可用于提高穿心莲叶片产量和诱导有效成分的产生。
Cytokinins are phytohormones that play multiple roles to control plant growth and development. In this study, leaf biomass and the production of andrographolide compounds in a medicinal plant Andrographis paniculata were significantly increased after exogenously treating with the synthetic cytokinin cytokinin-1-(2-chloro-4-pyridyl)-3-phenylurea (CPPU) at 0 (water), 5, or 10 mg L−1 and observed the results for 24 h, 48 h, and 7 days of treatment. It was found that CPPU could significantly enhance new axillary bud formation and further promote branching 4.6–5.6-fold higher, resulting in higher fresh weight (FW) and dry weight (DW) than the control. Application of CPPU at 5 mg L−1 significantly promoted the highest contents of total reducing sugar at 2.5-fold in leaves and at 1.5-fold in roots. Although treatments of CPPU significantly affected the increasing contents of chlorophyll and carotenoid (1.2–1.6-fold), CPPU at 10 mg L−1 slightly caused leaf stress and chlorophyll reduction. Interestingly, 5 mg L−1 CPPU could enhance andrographolide content, an active anti-infectious compound in Andrographis paniculata (2.2-fold higher than the control) that reached the highest content at 24 h after treatment. This study suggested that CPPU should be suitable for field application to promote leaf yields and induce the production of useful pharmaceutical compounds in Andrographis paniculata.
利什曼原虫分化过程中微管蛋白生物合成的转录后控制。
影响因子：
64.8
作者：
Wallach,M;Fong,D;Chang,KP
通讯作者：
Chang,KP