Epstein-Barr virus gene polymorphism in different parts of the same nasopharyngeal carcinoma patient
Epstein-Barr virus gene polymorphism in different parts of the same nasopharyngeal carcinoma patient
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同一鼻咽癌患者不同部位EB病毒基因多态性分析
DOI：
10.1007/s00705-012-1578-2
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发表时间：
2013-05-01
影响因子：
2.7
通讯作者：
Luo, Bing
中科院分区：
文献类型：
--
作者：
Nie, Yahong;Sun, Yingjuan;Luo, Bing
在以往的研究中，EB病毒（EBV）基因多态性和基因型的比较，从肿瘤和正常的咽喉冲洗液（TW）从不同的个人之间的菌株。然而，不同亚型的EB病毒是否存在于同一NPC患者的不同部位仍存在争议。为了解决这一问题，本研究比较了20例鼻咽癌患者的成对咽洗液和石蜡包埋组织中的基因型，发现大多数情况下同一个体的咽洗液和肿瘤细胞中的基因型相同。EBER和EBNA1基因的亚型进一步测序，并在肿瘤组织和TW中鉴定出相同的EBV株。总之，同一个体的不同部位感染相同的EBV毒株，除了偶尔的情况下，有一些差异，这表明在咽喉洗涤液中检测到的EBV亚型是一个合理的指导亚型存在于癌组织。
In most previous studies, comparisons of Epstein-Barr virus (EBV) gene polymorphisms and genotypes were made between strains from tumors and normal throat washings (TWs) from different individuals. However, it remains controversial whether different EBV subtypes are present in different parts of the same NPC patient. In order to address this question, in this study, we compared the genotypes in sets of paired throat washings (TW) and paraffin-embedded tissues of 20 patients with nasopharyngeal carcinoma (NPC), and we found the same genotype in throat washings (TWs) and tumor cells from the same individual in most cases. The subtypes of EBER and EBNA1 genes were furthermore sequenced, and identical EBV strains were identified in tumor tissues and TWs. In conclusion, different sites of the same individual are infected by the same EBV strains, except for a few differences in occasional cases, suggesting that the EBV subtype detected in throat washings is a reasonable guide to the subtype present in the carcinoma tissue.