A fast and simple analysis of glyphosate in tea beverages by capillary electrophoresis with on-line copper(II)-glyphosate complex formation
A fast and simple analysis of glyphosate in tea beverages by capillary electrophoresis with on-line copper(II)-glyphosate complex formation
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DOI：
10.1248/jhs.54.602
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发表时间：
2008-10-01
影响因子：
--
通讯作者：
Hayakawa, Kazuichi
中科院分区：
文献类型：
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作者：
Kodama, Shuji;Ito, Yuji;Hayakawa, Kazuichi
草甘膦是一种用于许多植物的除草剂，在高剂量下可能对人体有毒。目前测量草甘膦的方法既缓慢又昂贵。本文介绍了一种利用毛细管电泳在线生成铜(Il)-草甘膦络合物，快速简便地测定茶饮料中草甘膦含量的方法。最佳运行条件为含5 mM CUSO(4)的40mM醋酸缓冲液（pH 5.0），有效电压为+15 kV，使用磺化毛细管（FunCap-CE Type S），直接紫外检测波长为250 nm。草甘膦在5 ~ 1000 mg/l范围内呈线性关系（r(2) > 0.999）。峰面积（相对标准偏差< 1.2%）和迁移时间（相对标准偏差< 0.2%）重现性好。草甘膦回收率在98 ~ 100%之间。用该方法对加入草甘膦制剂的茶饮料进行了分析。
Glyphosate is an herbicide used for many plants that can be toxic to humans in high doses. Current methods for measuring glyphosate are slow and expensive. Here we describe a fast and simple method for measuring glyphosate in tea beverages by capillary electrophoresis with on-line formation of copper(Il)-glyphosate complex. The optimum running conditions were found to be 40mM acetate buffer (pH 5.0) containing 5 mM CUSO(4) with an effective voltage of +15 kV using a sulfonated capillary (FunCap-CE Type S) and direct UV detection at 250 nm. Linearity (r(2) > 0.999) was demonstrated in the range 5-1000 mg/l of glyphosate. Good reproducibilities of peak area (relative standard deviation < 1.2%) and migration time (relative standard deviation < 0.2%) were obtained. Recovery of glyphosate was between 98 and 100%. With this method, a tea beverage that was mixed with a glyphosate formulation was successfully analyzed.