EHRLICHIA MURIS SP-NOV, IDENTIFIED ON THE BASIS OF 16S RIBOSOMAL-RNA BASE SEQUENCES AND SEROLOGICAL, MORPHOLOGICAL, AND BIOLOGICAL CHARACTERISTICS
EHRLICHIA MURIS SP-NOV, IDENTIFIED ON THE BASIS OF 16S RIBOSOMAL-RNA BASE SEQUENCES AND SEROLOGICAL, MORPHOLOGICAL, AND BIOLOGICAL CHARACTERISTICS
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DOI：
10.1099/00207713-45-2-250
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发表时间：
1995-04-01
期刊：
影响因子：
--
通讯作者：
SUTO, C
中科院分区：
文献类型：
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作者：
WEN, BH;RIKIHISA, Y;SUTO, C
用PCR方法扩增了从日本野鼠分离的一株新病原体AS145（T）（T =模式株）的16SrRNA基因。采用TaqDNA聚合酶双脱氧核苷酸法直接测序。与埃里希体族和相关物种的其他成员的序列比较显示，从小鼠中分离的感染因子是埃里希体属的一个新物种，与美国人类埃里希体病的因子查菲埃里希体（Ehrlichia chaffeensis）（序列相似性水平为97.9%）最密切相关。该结果与用针对不同埃立克体病病原体的免疫血清进行的免疫印迹分析的结果一致。根据这些发现和其他形态学，生物学和血清学特征的有机体，我们建议，埃利希与这些属性属于一个新的物种，小鼠埃利希。
The 16S rRNA gene of a new infectious agent, strain AS145(T) (T = type strain), which was isolated from a wild mouse in Japan, was amplified by using the PCR. The amplimers were directly sequenced by dideoxynucleotide methods with Taq DNA polymerase. Sequence comparisons with other members of the tribe Ehrlichieae and related species revealed that the infectious agent isolated from the mouse is a new species of the genus Ehrlichia that is most closely related to Ehrlichia chaffeensis (level of sequence similarity, 97.9%), an agent of human ehrlichiosis in the United States. This result was consistent with the results of an immunoblot analysis performed with immune sera against different ehrlichiosis agents. On the basis of these findings and other morphological, biological, and serological characteristics of the organism, we propose that ehrlichiae with these properties belong to a new species, Ehrlichia muris.