Techniques to monitor glycolysis.
Techniques to monitor glycolysis.
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DOI：
10.1016/b978-0-12-416618-9.00005-4
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发表时间：
2014
影响因子：
--
通讯作者：
Teitell, Michael A.
中科院分区：
文献类型：
--
作者：
TeSlaa, Tara;Teitell, Michael A.
糖酵解增加的通量通过以ATP和葡萄糖衍生的代谢中间体的形式为核苷酸、脂肪和蛋白质的生物合成提供额外的能量，从而支持癌细胞的增殖。因此，糖酵解和其他控制细胞增殖的代谢途径可能是治疗干预和诊断程序的有价值的靶点。在这种背景下，测量恶性细胞对葡萄糖的摄取和乳酸的排泄可能有助于检测葡萄糖分解代谢的变化，而测定限速糖酵解酶的活性可以提供对代谢调节点的洞察。此外，代谢组学研究可以用来生成大型综合数据集，以跟踪糖酵解及其伴随的合成代谢途径中碳通量的变化。正如这里所讨论的，这些方法可以揭示和量化导致恶性细胞增殖的代谢变化。
An increased flux through glycolysis supports the proliferation of cancer cells by providing additional energy in the form of ATP as well as glucose-derived metabolic intermediates for nucleotide, lipid, and protein biosynthesis. Thus, glycolysis and other metabolic pathways that control cell proliferation may represent valuable targets for therapeutic interventions and diagnostic procedures. In this context, the measurement of glucose uptake and lactate excretion by malignant cells may be useful to detect shifts in glucose catabolism, while determining the activity of rate-limiting glycolytic enzymes can provide insights into points of metabolic regulation. Moreover, metabolomic studies can be used to generate large, integrated datasets to track changes in carbon flux through glycolysis and its collateral anabolic pathways. As discussed here, these approaches can reveal and quantify the metabolic alterations that underlie malignant cell proliferation.