Mutation of Rice Early Flowering3.1 (OsELF3.1) delays leaf senescence in rice
Mutation of Rice Early Flowering3.1 (OsELF3.1) delays leaf senescence in rice
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DOI：
10.1007/s11103-016-0507-2
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发表时间：
2016-07
影响因子：
5.1
通讯作者：
Yasuhito Sakuraba;Su-Hyun Han;Hyun-Jung Yang;Weilan Piao;N. Paek
中科院分区：
文献类型：
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作者：
Yasuhito Sakuraba;Su-Hyun Han;Hyun-Jung Yang;Weilan Piao;N. Paek
在拟南芥中，早期开花3（EARLY FLOWERING3, ELF3）在控制昼夜节律和光周期开花中起着关键作用。此外，ELF3通过抑制光敏色素相互作用因子4（phytochrome - interacting FACTOR4, PIF4）和光敏色素相互作用因子5（phytochrome - interacting FACTOR5, PIF5）的转录负向调控叶片衰老；elf3突变体比野生型（WT）衰老早，elf3过表达（ELF3-OX）植株衰老晚。在这里，我们发现，与抑制衰老的拟南芥ELF3相比，水稻同源体goself3.1促进叶片衰老；在黑暗诱导和自然衰老条件下，oself3.1突变体表现出延迟衰老，而doself3.1 - ox植株衰老更早。微阵列分析显示，在衰老叶片中，与WT相比，许多衰老相关基因、植物激素相关基因以及nac和wrky家族基因（OsNAP、ONAC106和doswrky42）在self3.1突变体中差异表达。有趣的是，我们发现过表达goself3.1的拟南芥植株与ELF3-OX植株一样，叶片衰老延迟，叶柄短，开花时间长。这表明elf3和doself3.1的调控功能在拟南芥和水稻之间是保守的，但下游的调控级联具有相反的作用。
InArabidopsis, EARLY FLOWERING3(ELF3) has pivotal roles in controlling circadian rhythm and photoperiodic flowering. In addition, ELF3 negatively regulates leaf senescence by repressing the transcription ofPHYTOCHROME-INTERACTING FACTOR4(PIF4) andPHYTOCHROME-INTERACTING FACTOR5(PIF5);elf3mutants senesce earlier andELF3-overexpressing (ELF3-OX) plants senesce later than wild type (WT). Here, we show that in contrast toArabidopsis ELF3, which represses senescence, the rice homologOsELF3.1promotes leaf senescence;oself3.1mutants showed delayed senescence andOsELF3.1-OX plants senesced earlier under both dark-induced and natural senescence conditions. Microarray analysis revealed that in the senescing leaves, a number of senescence-associated genes, phytohormone-related genes, andNACandWRKYfamily genes (OsNAP, ONAC106, andOsWRKY42) were differentially expressed inoself3.1mutants compared with WT. Interestingly, we found thatArabidopsisplants overexpressingOsELF3.1show delayed leaf senescence, produce short petioles, and flower late in long days, just likeArabidopsis ELF3-OX plants. This demonstrates that the regulatory functions ofELF3andOsELF3.1are conserved betweenArabidopsisand rice, but the downstream regulatory cascades have opposite effects.