Construction of a new set of rice chromosome segment substitution lines and identification of grain weight and related traits QTLs
Construction of a new set of rice chromosome segment substitution lines and identification of grain weight and related traits QTLs
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DOI：
10.1270/jsbbs.60.305
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发表时间：
2010-12-01
期刊：
影响因子：
2.4
通讯作者：
Wan, Jian Min
中科院分区：
文献类型：
--
作者：
Bian, Jian Min;Jiang, Ling;Wan, Jian Min
我们在这里报告了一组新的染色体片段置换系（CSSLs）的构建，其中优良粳稻品种C418的基因组已经渗入到优良籼稻品种9311的背景中。这套引物是利用136个SSR和in/del标记进行标记辅助选择而建立的。108个CSSLs中的渗入片段占C418基因组的98.3%。利用CSSL基因组分析了两种环境下千粒重及相关性状的遗传变异，共检测到41个数量性状位点，其中5个在两种环境下均表达。qTGW 7的更详细的定位显示，它与RM 22034共分离在7号染色体的短臂上。除了TGW之外，CSSLs在许多农艺性状方面表现出不同的表型。CSSL群体可以有效地鉴定这些性状的QTL，并为作物改良提供相关种质。
We report here the construction of a new set of chromosome segment substitution lines (CSSLs) in rice, in which the genome of the elite japonica cultivar C418 has been introgressed into the background of the elite indica cultivar 9311. This set was developed by marker aided selection, based on 136 SSR and in/del markers. The introgressed chromosomal segments presented in the 108 CSSLs covered 98.3% of the cultivar C418 genome. The CSSL set was used to genetically analyze variation for 1000-grain weight (TGW) and related traits in two contrasting environments, and led to the identification of 41 quantitative trait loci (QTLs), five of which were expressed in both environments. More detailed mapping of qTGW7 showed that it is cosegregated with RM22034 on the short arm of chromosome 7. The CSSLs varied phenotypically with respect to a number of agronomic traits besides TGW. CSSL populations would be effective in identifying QTLs for these various traits, and could provide germplasm relevant for crop improvement.