Three-dimensional visualization of tegument/capsid interactions in the intact human cytomegalovirus
Three-dimensional visualization of tegument/capsid interactions in the intact human cytomegalovirus
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DOI：
10.1006/viro.1999.9791
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发表时间：
1999-07-20
期刊：
影响因子：
3.7
通讯作者：
Zhou, ZH
中科院分区：
文献类型：
--
作者：
Chen, DH;Jiang, H;Zhou, ZH
用电子冷冻显微镜和计算机重建技术测定了完整的人巨细胞病毒（HCMV）的三维结构，其分辨率为18埃。它的衣壳壳由排列在T = 16二十面体晶格上的五邻体、六邻体和三链体组成，并且与从宿主细胞核分离的B-衣壳相同。还观察到由960个丝状密度拷贝形成的二十面体有序的皮层层，其与底层衣壳的五邻体、苍鹭和三联体相互作用。观察到的结构相似性和差异的HCMV与单纯疱疹病毒的感染过程中，同时保持类似的衣壳的不同的被膜成分的意义提供了见解。(C)北京：科学出版社.
The three-dimensional structure of the intact human cytomegalovirus (HCMV) was determined to 18-Angstrom resolution by electron cryomicroscopy and computer reconstruction. Its capsid shell is composed of pentons, hexons, and triplexes arranged on a T = 16 icosahedral lattice and is identical to that of the B-capsid isolated from host cell nuclei. An icosahedrally ordered tegument layer formed by 960 copies of filamentous density is also visualized, which interacts with the pentons, herons, and triplexes of the underlying capsid. The observed structural similarities and differences of HCMV with those of herpes simplex virus offer insights into the significance of the different tegument components for their infection processes while maintaining similar capsids. (C) 1999 Academic Press.