Synergistic interaction of ozone and respirable aerosols on rat lungs. II. Synergy between ammonium sulfate aerosol and various concentrations of ozone.
Synergistic interaction of ozone and respirable aerosols on rat lungs. II. Synergy between ammonium sulfate aerosol and various concentrations of ozone.
复制标题
臭氧和可吸入气溶胶对大鼠肺部的协同相互作用。
DOI：
10.1016/0041-008x(86)90252-8
复制
发表时间：
1986
影响因子：
3.8
通讯作者：
Last,JA
中科院分区：
文献类型：
--
作者：
Warren,DL;Guth,DJ;Last,JA
大鼠连续暴露于浓度为0.12至0.64 ppm的臭氧（O3）后的肺反应进行了定量，通过测量组织胶原合成率，组织蛋白质和DNA含量，以及支气管肺泡灌洗液的各种成分。暴露于0.64ppm的O3 7天后，肺胶原合成率和组织蛋白质和DNA含量升高。暴露于0.64 ppm O3的较短时间后，灌洗液中的蛋白质含量和乳酸脱氢酶、酸性磷酸酶和N-乙酰-β-D-氨基葡萄糖苷酶的活性显著升高。大鼠暴露于0.20 ppm的O3 7天后，肺胶原合成率和组织蛋白含量均发生变化。总lavagable蛋白质含量，一个敏感的指标O3对肺的诱导作用，显着升高暴露于0.12或0.20 ppm的O3大鼠的肺。要检查是否发生协同作用之间的0.20或0.64 ppm的O3和酸性气溶胶，大鼠连续暴露于O3与和不同时暴露于5毫克/立方米的硫酸铵。通过测量总可灌洗蛋白和肺胶原合成率，观察到0.20 ppm O3和硫酸铵气溶胶之间的协同相互作用。这些结果表明，硫酸铵气溶胶相互作用协同O3浓度的O3接近环境水平。
Pulmonary responses after continuous exposure of rats to concentrations of ozone (O3) ranging from 0.12 to 0.64 ppm were quantified by measuring tissue collagen synthesis rate, tissue protein and DNA content, and various constituents of bronchoalveolar lavage fluid. After 7 days of exposure to 0.64 ppm of O3, lung collagen synthesis rate and tissue content of protein and DNA were elevated. After shorter durations of exposure to 0.64 ppm of O3, significant elevations were observed in the protein content and the activities of lactate dehydrogenase, acid phosphatase, and N-acetyl-β-d-glucosaminidase from lavage fluid. After exposure of rats to 0.20 ppm of O3for 7 days, changes could be detected in both lung collagen synthesis rate and tissue protein content. Total lavagable protein content, a sensitive indicator for O3-induced effects upon the lung, was significantly elevated in lungs of rats exposed to 0.12 or 0.20 ppm of O3. To examine whether a synergistic interaction occurred between 0.20 or 0.64 ppm of O3and acid aerosols, rats were continuously exposed to O3with and without concurrent exposure to 5 mg/m3of ammonium sulfate. A synergistic interaction between 0.20 ppm of O3and ammonium sulfate aerosol was observed by measurement of total lavagable protein and of lung collagen synthesis rate. These results demonstrate that ammonium sulfate aerosol interacts synergistically with O3at concentrations of O3that approach ambient levels.