Transcriptome changes favoring intramuscular fat deposition in the longissimus muscle following castration of bulls
Transcriptome changes favoring intramuscular fat deposition in the longissimus muscle following castration of bulls
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DOI：
10.2527/jas.2012-6089
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发表时间：
2013-10-01
影响因子：
3.3
通讯作者：
Baik, M.
中科院分区：
文献类型：
--
作者：
Jeong, J.;Bong, J.;Baik, M.
阉割增加肌内脂肪（IMF）沉积，提高牛肉质量的牛。本研究旨在确定公牛去势后的全球转录组变化，并确定与韩国牛背最长肌（LM）IMF沉积相关的基因。构建定制的牛CombiMatrix寡核苷酸微阵列，并通过微阵列杂交确定去势后的转录组变化。公牛和阉牛之间的转录组比较表明，8,407个基因中有428个在LM中差异表达超过2倍（P < 0.05）。基因表达谱分析表明，在几个途径，包括脂肪形成，脂肪酸氧化，三羧酸（TCA）循环，氧化磷酸化（OP），去势后的改变。去势上调了成脂周脂蛋白2（PLIN 2）和内脂素、脂肪酸合成酶、脂肪酸酯化1-酰基甘油-3-磷酸O-酰基转移酶5和许多脂肪酸氧化相关基因的转录。许多TCA循环和OP基因也转录上调。相关分析表明，LM中IMF含量与PLIN 2 mRNA水平高度相关（r = 0.70，P < 0.001），腺苷三磷酸酶（ATP酶），H+转运，溶酶体42 kDa，V1亚基C1（ATP 6V 1C 1：r = 0.66，P < 0.001），和细胞色素c氧化酶组装同源物11（COX11：r = 0.72，P < 0.001）基因，在单独饲养组中，PLIN 2和PLIN 3基因保持显著相关性，ATP 6V1C1（r = 0.50，P <0.05）和COX 11（r = 0.60，P < 0.01）。总之，我们的研究提供的证据表明，去势转移脂质代谢基因的转录，有利于IMF沉积增加脂肪生成，脂肪生成和甘油三酯的合成。这项研究还表明，阉割增加了参与脂肪酸氧化和随后的能量生产（TCA和OP基因）的基因的转录。我们的微阵列分析提供了新的信息，阉割改变了与脂质/能量代谢相关的转录组，有利于LM中的IMF沉积。
Castration increases intramuscular fat (IMF) deposition, improving beef quality in cattle. The present study was performed to determine the global transcriptome changes following castration of bulls and to identify genes associated with IMF deposition in the longissimus dorsi (LM) of Korean cattle. A customized bovine CombiMatrix oligonucleotide microarray was constructed, and transcriptome changes following castration were determined by microarray hybridization. Transcriptome comparison between bulls and steers indicated that 428 of 8,407 genes were differentially expressed in the LM by greater than two fold (P < 0.05). Gene expression profiling indicated alterations in several pathways, including adipogenesis, fatty acid oxidation, tricarboxylic acid (TCA) cycle, and oxidative phosphorylation (OP), following castration. Castration upregulated transcription of adipogenic perilipin 2 (PLIN2) and visfatin, lipogenic fatty acid synthase, fatty acid esterification 1-acylglycerol-3-phosphate O-acyltransferase 5, and many fatty acid oxidation-related genes. Many TCA cycle and OP genes were also transcriptionally upregulated. Correlation analysis indicated that the IMF content in the LM was highly correlated with mRNA levels of PLIN2 (r = 0.70, P < 0.001), adenosine triphosphatase (ATPase), H+-transporting, lysosomal 42 kDa, V1 subunit C1 (ATP6V1C1: r = 0.66, P < 0.001), and cytochrome c oxidase assembly homolog 11 (COX11: r = 0.72, P < 0.001) genes in a pooled animal group of steers plus bulls, and significant correlations in the steer-alone group were maintained in the 3 genes, PLIN2 (r = 0.47, P < 0.05), ATP6V1C1 (r = 0.50, P < 0.05), and COX11 (r = 0.60, P < 0.01). In conclusion, our study provided evidence that castration shifts transcription of lipid metabolism genes, favoring IMF deposition by increasing adipogenesis, lipogenesis, and triglyceride synthesis. This study also indicated that castration increases transcription of genes involved in fatty acid oxidation and subsequent energy production (TCA and OP genes). Our microarray analysis provided novel information that castration alters the transcriptome associated with lipid/energy metabolism, favoring IMF deposition in the LM.