Labelling the hydrophobic core of membranes
Labelling the hydrophobic core of membranes
复制标题
标记膜的疏水核心
DOI：
10.1016/0968-0004(81)90017-7
复制
发表时间：
1981
影响因子：
13.8
通讯作者：
J. Brunner
中科院分区：
文献类型：
--
作者：
J. Brunner
膜疏水标记的目的是鉴定嵌入在脂质核心中的膜蛋白的结构域。通过使用能够在膜的极性和惰性脂相内反应的光反应试剂，该技术是对现有膜表面标记方法的有益补充。在过去的几年里，一种有机化学方法已经被开发出来，专门标记那些穿透生物膜的脂质基质的完整膜蛋白的结构域。在本文中，讨论了疏水标记的一些主要方面，并比较了用于此目的的各种类型的试剂。
The aim of hydrophobic labelling of membranes is to identify those domains of membrane proteins embedded in the lipid core. By using photoreactive reagents capable of reacting within the apolar and mostly inert lipid phase of membranes, this technique is a useful complement to existing methods for labelling membrane surface.During the past few years, an organochemical approach has been developed to specifically label those domains of integral membrane proteins that penetrate the lipid matrix of a biological membrane. In this article some of the principal aspects of hydrophobic labelling are discussed and the various types of reagents used for this purpose are compared.