O2 regulates surfactant protein A mRNA transcription and stability in human fetal lung in vitro.
O2 regulates surfactant protein A mRNA transcription and stability in human fetal lung in vitro.
复制标题
O2 在体外调节人胎肺中表面活性蛋白 A mRNA 的转录和稳定性。
DOI：
10.1152/ajplung.1998.274.3.l343
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Snyder,JM
中科院分区：
文献类型：
--
作者：
Acarregui,MJ;Kumar,AR;Penisten,ST;Snyder,JM
用20%和70%O2培养中期人胎肺组织，观察O2对肺表面活性蛋白(SP)A基因转录和半衰期的影响。组织在70%O2中孵育后，SP-A mRNA转录速率较对照组织高133%。肺组织在70%O2中培养，SP-A mRNA半衰期延长54%。控制组织。Western印迹分析显示，在70%O2状态下，SP-A的表达增加了3倍，说明O2对SP-A的mRNA水平的调节导致了SP-A水平的相应变化。进行了引物延伸分析，以确定观察到的SP-A mRNA水平的增加是否是人类SP-A基因之一SP-A1或SP-A2优先表达的次要因素。在70%氧气条件下，SP-A1和SP-A2基因的转录水平均较对照组织高出100%。∼结果显示，在70%氧气中维持的组织中，SP-A1和SP-A2基因的转录水平均高于对照组织。这些数据表明，O2通过转录和转录后机制调节人SP-A的mRNA水平。此外，由于O2对SP-A1和SP-A2基因的表达没有不同的影响，所以这些介导O2调节的基因的性质必须在这两个基因之间保守。
The effect of O2on surfactant protein (SP) A mRNA transcription and half-life was determined in midtrimester human fetal lung tissue cultured in either 20 (control) or 70% O2. Incubation of tissues in 70% O2resulted in a 133% increase in SP-A mRNA transcription rate compared with control tissues. The SP-A mRNA half-life was increased by 54% in lung tissues cultured in 70% O2vs. control tissues. Western blot analysis indicated a threefold increase in SP-A in the 70% O2condition, demonstrating that O2regulation of SP-A mRNA levels results in corresponding changes in SP-A levels. Primer extension assays were performed to determine whether the observed increase in SP-A mRNA levels is secondary to the preferential expression of one of the human SP-A genes, SP-A1 or SP-A2. Transcripts of both the SP-A1 and SP-A2 genes were increased ∼100% in tissues maintained in 70% O2compared with control tissues. These data demonstrate that O2regulates human SP-A mRNA levels by both transcriptional and posttranscriptional mechanisms. Furthermore, because there is no differential effect of O2on the expression of SP-A1 vs. SP-A2 mRNA, the properties of these genes that mediate regulation by O2must be conserved between the two genes.