Development of a 3D Collagen Scaffold Coated With Multiwalled Carbon Nanotubes
Development of a 3D Collagen Scaffold Coated With Multiwalled Carbon Nanotubes
复制标题
DOI：
10.1002/jbm.b.31327
复制
发表时间：
2009-08-01
影响因子：
3.4
通讯作者：
Yokoyama, Atsuro
中科院分区：
文献类型：
--
作者：
Hirata, Eri;Uo, Motohiro;Yokoyama, Atsuro
碳纳米管（CNTs）具有良好的生物化学特性，如细胞粘附性和蛋白质吸附性，是一种非常有用的细胞培养支架材料。本研究制备了一种多壁碳纳米管包覆的胶原海绵（MWCNT-coated sponge，MWCNT-coated sponge），以改善胶原海绵的表面性质，并对其细胞培养性能进行了检测。胶原海绵表面通过分散的方式均匀包覆多壁碳纳米管。MC 3 T3-E1细胞在MWCNT包被的海绵上和海绵内培养。培养1周后，MWCNT涂覆的海绵上的DNA含量显著高于未涂覆的胶原海绵上的DNA含量（p < 0.05）。在MWCNT包被的海绵和未包被的胶原海绵（众所周知是细胞培养的最佳支架之一）上通过DNA量归一化的估计ALP活性之间没有显著差异。此外，MWCNT涂覆的表面显示出强的细胞粘附。因此，多壁碳纳米管涂层的胶原蛋白海绵有望成为一种有用的细胞培养三维支架。(C)2009 Wiley Periodicals，Inc.生物医学材料研究杂志B部分：应用生物材料90 B：629-634，2009
Carbon nanotubes (CNTs) have attractive biochemical properties such as strong cell adhesion and protein absorption, which are very useful for a cell cultivation scaffold. In this study, We prepared a multiwalled carbon nanotube-coated collagen sponge (MWCNT-coated sponge) to improve the surface properties of the collagen sponge, and its cell culturing properties Were examined. The suface of the collagen sponge was homogeneously coated with MWCNTs by dispersion. MC3T3-E1 cells were cultured on and inside the MWCNT-coated sponge. The DNA content on the MWCNT-coated sponge after 1 week of culture was significantly higher than on an uncoated collagen sponge (p < 0.05). There was no significant difference between the estimated ALP activity normalized by DNA quantity on the MWCNT-coated sponge and that on the uncoated collagen sponge which is well known as one of the best scaffolds for cell cultivation. In addition, the MWCNT-coated surface shows strong cell adhesion. Therefore, the MWCNT-coated collagen sponge is expected to be a useful 3D scaffold for cell cultivation. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 90B: 629-634, 2009