Differential expression of the Cs and Cα1 isoforms of the catalytic subunit of cyclic 3′,5′-adenosine monophosphate-dependent protein kinase in testicular cells
Differential expression of the Cs and Cα1 isoforms of the catalytic subunit of cyclic 3′,5′-adenosine monophosphate-dependent protein kinase in testicular cells
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DOI：
10.1095/biolreprod65.1.151
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发表时间：
2001-07-01
影响因子：
3.6
通讯作者：
Witman, GB
中科院分区：
文献类型：
--
作者：
San Agustin, JT;Witman, GB
精子cAMP依赖性蛋白激酶催化亚基（称为C-s）的氨基末端与大多数组织中表达的C α 1亚型不同，这是由于使用了C α基因的替代转录本。C α 1和C-s转录本都存在于睾丸中; C-s在生精细胞中特异性表达，并且是在成熟精子中检测到的唯一C亚型。使用C-S和Ccxl特异性抗体的小鼠睾丸免疫组织化学现在表明，Cal存在于睾丸体细胞、精原细胞和前细线期精母细胞中，但不存在于精子发生后期的细胞中。相反，C-s仅在粗线期中期和后期精母细胞和精子细胞中表达。因此，C-s和C α 1表达不重叠。免疫荧光显微镜定位的C-S在小鼠和绵羊精子显示，C-S主要位于精子尾部组件，包括中段线粒体和轴丝。蛋白质印迹的定量分析表明，单个绵羊精子含有大约4 × 10（5）个C-s分子，对于一种起催化作用的蛋白质来说，这似乎是一个很大的数目。
The amino terminus of the sperm cAMP-dependent protein kinase catalytic subunit (termed C-s) differs from that of the C alpha1 isoform expressed in most tissues due to the use of alternative transcripts of the C alpha gene. Both C alpha1 and C-s transcripts are present in testis; C-s is expressed specifically in spermatogenic cells and is the only C isoform detected in mature sperm. Immunohistochemistry of mouse testis using antibodies specific for C-s and Ccxl now shows that Cal is present in somatic testicular cells, spermatogonia, and preleptotene spermatocytes but not in cells that are in later stages of spermatogenesis. In contrast, C-s is expressed only in midpachytene and later stage spermatocytes and in spermatids. Therefore, C-s and C alpha1 expression do not overlap. Immunofluorescence microscopic localization of C-s in murine and ovine sperm reveals that C-s is located primarily in sperm tail components, including the midpiece mitochondria and the axoneme. Quantitative analysis of Western blots indicates that individual ovine sperm contain similar to4 x 10(5) molecules of C-s, a seemingly large number for a protein that acts catalytically.