Cryo-electron tomography of porcine reproductive and respiratory syndrome virus: organization of the nucleocapsid
Cryo-electron tomography of porcine reproductive and respiratory syndrome virus: organization of the nucleocapsid
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DOI：
10.1099/vir.0.007674-0
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发表时间：
2009-03-01
影响因子：
3.8
通讯作者：
Dokland, Terje
中科院分区：
文献类型：
--
作者：
Spilman, Michael S.;Welbon, Craig;Dokland, Terje
猪繁殖与呼吸道病毒 (PRRSV) 是动脉病毒科的一种有包膜正义 RNA 病毒，可在全世界范围内引起猪的严重且持续的疾病。 PRRSV 病毒粒子由脂质包膜组成，脂质包膜含有多个包膜蛋白，包膜蛋白围绕着核衣壳核心，核衣壳核心包裹着 RNA 基因组。为了更好地了解 PRRSV 的结构和组装，我们对 MARC-145 细胞中生长的病毒粒子进行了冷冻电子显微镜和断层扫描重建。病毒体为多形性、圆形至蛋形颗粒，平均直径为 58 nm。这些颗粒显示出光滑的外表面，只有一些突出的特征，可能与包膜蛋白复合物相对应。病毒粒子包含平均直径为 39 nm 的双层中空核心，其与包膜之间有 2-3 nm 的间隙。对三维结构的分析表明，核心由捆绑成空心球的双层核衣壳蛋白链组成。
Porcine reproductive and respiratory virus (PRRSV) is an enveloped positive-sense RNA virus of the family Arteriviridae that causes severe and persistent disease in pigs worldwide. The PRRSV virion consists of a lipid envelope that contains several envelope proteins surrounding a nucleocapsid core that encapsidates the RNA genome. To provide a better understanding of the structure and assembly of PRRSV, we have carried out cryo-electron microscopy and tomographic reconstruction of virions grown in MARC-145 cells. The virions are pleomorphic, round to egg-shaped particles with an average diameter of 58 nm. The particles display a smooth outer surface with only a few protruding features, presumably corresponding to the envelope protein complexes. The virions contain a double-layered, hollow core with an average diameter of 39 nm, which is separated from the envelope by a 2-3 nm gap. Analysis of the three-dimensional structure suggests that the core is composed of a double-layered chain of nucleocapsid proteins bundled into a hollow ball.