Calcium and calmodulin-dependent protein phosphorylation in rabbit ileum.
Calcium and calmodulin-dependent protein phosphorylation in rabbit ileum.
复制标题
兔回肠中钙和钙调蛋白依赖性蛋白磷酸化。
DOI：
10.1016/0014-5793(81)80395-x
复制
发表时间：
1981
期刊：
影响因子：
3.5
通讯作者：
Sharp,GW
中科院分区：
文献类型：
--
作者：
Taylor,L;Guerina,VJ;Donowitz,M;Cohen,M;Sharp,GW
越来越多的证据表明，钙是肠道电解质转运的生理调节剂[1-3]。增加细胞内钙的条件，如使用钙离子载体A23187 [1，2]或暴露于神经体液物质，如血清素[4]或卡巴胆碱[1]，可刺激肠氯分泌和/或抑制Na+和Cl-吸收。相反，降低细胞内钙的条件，如暴露于钙通道阻滞剂维拉帕米，刺激Na+和Cl-吸收[3]。钙调蛋白可能参与这些作用，因为抗精神病药物三氟拉嗪（钙-钙调蛋白复合物的抑制剂）抑制由钙离子载体A23187引起的肠道分泌[5，6]。钙和钙调素影响肠离子转运的细胞内机制尚不清楚。然而，由于钙和钙调蛋白影响其他几个系统中特定蛋白质的磷酸化和功能[7，8]，磷酸化是回肠电解质转运的潜在控制机制。这些研究首次证明了钙和钙调蛋白可以引起肠肽的磷酸化。
Evidence is accumulating to suggest that calcium is a physiological regulator of intestinal electrolyte transport [1-3]. Conditions which increase intracellular calcium such as the use of the calcium ionophore A23187 [1, 2] or exposure to neurohumoral substances such as serotonin [4] or carbachol [1] cause stimulation of intestinal chloride secretion and/or inhibition ofNa÷ and C1-absorption. To the contrary, conditions which decrease intracellular calcium, such as exposure to the calcium channel blocker verapamil, stimulate Na÷ and C1-absorption [3]. Calmodulin may be involved in these effects since the anti-psychotic drug trifluoperazine (an inhibitor of the calcium-calmodulin complex) inhibited intestinal secretion caused by the calcium ionophore A23187 [5, 6]. The intracellular mechanisms by which calcium and calmodulin affect intestinal ion transport are not known. However, as calcium and calmodulin affect phosphorylation and function of specific proteins in several other systems [7, 8], phosphorylation is a potential control mechanism for ileal electrolyte transport. These studies are the first demonstration that calcium and calmodulin can cause phosphorylation of intestinal peptides.