Effect of cyclic mechanical loading on osteoclast recruitment in periodontal tissue.
Effect of cyclic mechanical loading on osteoclast recruitment in periodontal tissue.
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DOI：
10.1111/j.1600-0765.2008.01193.x
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发表时间：
2010-02
影响因子：
3.5
通讯作者：
K. Nozaki;M. Kaku;Y. Yamashita;M. Yamauchi;H. Miura
中科院分区：
文献类型：
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作者：
K. Nozaki;M. Kaku;Y. Yamashita;M. Yamauchi;H. Miura
背景与目的周期性机械负荷诱导牙周组织破骨细胞生成已被广泛接受，但其分子机制尚不清楚，部分原因是缺乏合适的模型。在这项研究中，我们研究了一种新的设备，允许循环机械负载，以良好的控制方式进行。此外，通过使用该模型，周期性负荷对破骨细胞在牙周组织中的募集的影响进行了描述。材料与方法采用自行研制的装置，对大鼠上颌右磨牙分别施加20 n（参考载荷，对应于膳食颗粒的断裂硬度）和两种过度载荷（30和40 n），持续7 d，通过免疫组化分析相关标志蛋白，评价破骨细胞在牙周组织中的募集情况。结果30 N压力作用3 d后，牙槽骨内破骨细胞形成;在30和40 N负荷下，抗酒石酸酸性磷酸盐、核因子-κ B受体激活剂配体和骨保护素阳性的细胞在牙槽骨/牙周韧带中以时间依赖性方式显著增加。结论：开发了一种允许施加不同水平循环机械载荷的新器械。通过在大鼠中使用该装置，观察到牙周组织中破骨细胞募集的早期事件，以时间依赖性的方式过度负荷，表明该模型的有用性。
BACKGROUND AND OBJECTIVE It is well accepted that cyclic mechanical loading induces osteoclastogenesis in periodontal tissue, but its molecular mechanisms are not well understood, in part because of a lack of appropriate models. In this study, we investigated a novel device that allows cyclic mechanical loading to be performed in a well-controlled manner. Furthermore, by employing this model, the effect of cyclic loading on osteoclast recruitment in the periodontal tissue was described. MATERIAL AND METHODS By using a newly developed device, the cyclic loading of 20 n (reference loading corresponding to the fracture hardness of dietary pellets) and two excessive loadings (i.e. 30 and 40 n) were applied to maxillary right molars in rats for up to 7 d, and osteoclast recruitment in the periodontal tissue was evaluated by analyzing relevant marker proteins using immunohistochemistry. RESULTS Osteoclastogenesis was induced by day 3 within alveolar bone subjected to a compression force of 30 n. With both 30 and 40 n loadings, cells that were positive to for tartrate-resistant acid phosphate, receptor activator of nuclear factor-kappaB ligand and osteoprotegerin were significantly increased in the alveolar bone/periodontal ligament in a time-dependent manner. CONCLUSION A new device was developed that allows various levels of cyclic mechanical loading to be exerted. By using this device in rats, early events of osteoclast recruitment in the periodontal tissues were observed with excessive loadings in a time-dependent manner, indicating the usefulness of this model.