Leucine kinetics in endurance-trained humans.
Leucine kinetics in endurance-trained humans.
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经过耐力训练的人类的亮氨酸动力学。
DOI：
10.1152/jappl.1990.69.1.1
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Kalhan,SC
中科院分区：
文献类型：
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作者：
Lamont,LS;Patel,DG;Kalhan,SC
这项研究比较了耐力训练（TRN）和久坐（SED）对照组的全身亮氨酸动力学。11名男性和女性（6名TRN， 5名SED）接受了6小时的L-[1-13C]亮氨酸的等速输注。用示踪剂稀释法和测定过期CO2的13C富集量法结合呼吸量热法测定亮氨酸的周转和氧化。TRN组的全身亮氨酸转化率更高（P < 0.004； TRN为98.3 +/- 5.0，SED为75.3 +/- 4.2，平均+/- SE），但亮氨酸氧化组间无差异（TRN为13.1 +/- 0.97，SED为11.5 +/- 0.48,mumol.kg-1.h-1）。因此，更多的亮氨酸可用于非氧化利用。此外，TRN受试者的静息能量消耗高于SED组，当所有受试者纳入分析时，能量消耗与蛋白质周转之间存在显著相关性（n = 11, R = 0.61, P = 0.05）。因此，TRN受试者的静息能量消耗增加可能是由全身蛋白质周转增加引起的。这些结果表明，耐力训练导致亮氨酸和/或蛋白质周转增加，这可能有助于这些受试者观察到的静息能量消耗增加。
This study compared whole-body leucine kinetics in endurance-trained (TRN) and sedentary (SED) control subjects. Eleven men and women (6 TRN, 5 SED) underwent a 6-h primed, constant-rate infusion of L-[1-13C]leucine. Leucine turnover and oxidation were measured using tracer dilution and by measuring 13C enrichment of expired CO2 combined with respiratory calorimetry. Whole-body leucine turnover was greater in the TRN subjects (P less than 0.004; TRN 98.3 +/- 5.0, SED 75.3 +/- 4.2 mumol.kg-1.h-1; mean +/- SE), but there was no difference between groups in leucine oxidation (TRN 13.1 +/- 0.97, SED 11.5 +/- 0.48 mumol.kg-1.h-1). Thus more leucine turnover was available for nonoxidative utilization. In addition, the TRN subjects had higher resting energy expenditures compared with the SED group, and when all subjects were included in the analysis, there was a significant correlation between energy expenditure and protein turnover (n = 11, R = 0.61, P = 0.05). Therefore the heightened resting energy expenditure in the TRN subjects may be accounted for by an increased whole-body protein turnover. These results suggest that endurance training results in increased leucine and/or protein turnover, which may contribute to the increased resting energy expenditure observed in these subjects.